TAILIEUCHUNG - Báo cáo khoa học: Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegelii – environmental adaptation of the hatching enzyme and evolutionary aspects of formation of the pseudogene

The hatching enzyme of oviparous euteleostean fishes consists of two metalloproteases: high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE). They cooperatively digest the egg envelope (chorion) at the time of embryo hatching. In the present study, we investigated the hatching of embryos of the ovoviviparous black rockfish Sebastes schlegelii. | ễFEBS Journal Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegelii - environmental adaptation of the hatching enzyme and evolutionary aspects of formation of the pseudogene Mari Kawaguchi1 Masahiro Nakagawa2 Tsutomu Noda3 Norio Yoshizaki4 Junya Hiroi5 Mutsumi Nishida6 Ichiro Iuchi1 and Shigeki Yasumasu1 1 Life Science Institute Sophia University Tokyo Japan 2 NationalCenter for Stock Enhancement Fisheries Research Agency Goto Station Nagasaki Japan 3 NationalCenter for Stock Enhancement Fisheries Research Agency Miyako Station Iwate Japan 4 Department of AnimalResource Production United Graduate Schoolof AgriculturalScience Gifu University Japan 5 Department of Anatomy St Marianna University Schoolof Medicine Kawasaki Japan 6 Ocean Research Institute University of Tokyo Japan Keywords aberrant splicing adaptation astacin family metalloprotease hatching enzyme pseudogene Correspondence S. Yasumasu Life Science Institute Sophia University 7-1 Kioi-cho Chiyoda-ku Tokyo 102-8554 Japan Fax 81 3 3238 3393 Tel 81 3 3238 4263 E-mail s-yasuma@ Database The nucleotide sequence data have been submitted to the DDBJ EMBL GenBank nucleotide sequence databases under the accession numbers AB353099-AB353111 Received 17 February 2008 revised 25 March 2008 accepted 1 April 2008 doi The hatching enzyme of oviparous euteleostean fishes consists of two metalloproteases high choriolytic enzyme HCE and low choriolytic enzyme LCE . They cooperatively digest the egg envelope chorion at the time of embryo hatching. In the present study we investigated the hatching of embryos of the ovoviviparous black rockfish Sebastes schlegelii. The chorion-swelling activity HCE-like activity was found in the ovarian fluid carrying the embryos immediately before the hatching stage. Two kinds of HCE were partially purified from the fluid and the relative molecular masses of them matched well with those deduced from two HCE cDNAs .

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