TAILIEUCHUNG - Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability

Aeropyrum pernixK1, an aerobic hyperthermophilic archaeon, produces a cambialistic superoxide dismutase that is active in the presence of either of Mn or Fe. The crystal structures of the superoxide dismutase fromA. per-nixin the apo, Mn-bound and Fe-bound forms were determined at resolu-tions of , and A ˚ , respectively. | IFEBS Journal Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 - insights into the enzyme mechanism and stability Tsutomu Nakamura1 Kasumi Torikai1 2 Koichi Uegaki1 Junji Morita2 Kodai Machida3 4 Atsushi Suzuki5 and Yasushi Kawata3 4 1 National institute of Advanced Industrial science and Technology Ikeda Osaka Japan 2 Department of Food Science and Nutrition Faculty of Human Life and Science Doshisha Women s College of LiberalArts Kyoto Japan 3 Department of Chemistry and Biotechnology Graduate Schoolof Engineering Tottori University Japan 4 Department of BiomedicalScience Institute of Regenerative Medicine and Biofunction Graduate Schoolof MedicalScience Tottori University Japan 5 Power Train MaterialEngineering Division Toyota Motor Corporation Aichi Japan Keywords Aeropyrum pernix cambialistic metal coordination stability superoxide dismutase Correspondence T. Nakamura National institute of Advanced IndustrialScience and Technology 1-8-31 Midorigaoka Ikeda Osaka 563-8577 Japan Fax 81 72 751 8370 Tel 81 72 751 9272 E-mail nakamura-t@ Received 2 August 2010 revised 28 October 2010 accepted 2 December 2010 doi Aeropyrum pernix K1 an aerobic hyperthermophilic archaeon produces a cambialistic superoxide dismutase that is active in the presence of either of Mn or Fe. The crystal structures of the superoxide dismutase from A. per-nix in the apo Mn-bound and Fe-bound forms were determined at resolutions of and A respectively. The overall structure consisted of a compact homotetramer. Analytical ultracentrifugation was used to confirm the tetrameric association in solution. In the Mn-bound form the metal was in trigonal bipyramidal coordination with five ligands four side chain atoms and a water oxygen. One aspartate and two histidine side chains ligated to the central metal on the equatorial plane. In the Fe-bound form an additional water molecule was observed between the two .

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