TAILIEUCHUNG - Improvement of pearl millet (Pennisetum glaucum (l.) r. br) prolamin extractability: Chromatographic separation, characterization and functional properties

The major protein fraction in pearl millet is prolamin the alcohol soluble fraction called pennisetin. Researches on the factors affecting pennisetin extraction and on its functional properties are still very scant. In this paper, the effect of temperature, reducing agents (β-mercaptoethanol, dithiothreitol or sodium metabisulfite) and sodium hydroxide on pennisetin extraction was assessed. | IMPROVEMENT OF PEARL MILLET Pennisetum glaucum L. R. BR PROLAMIN EXTRACTABILITY CHROMATOGRAPHIC SEPARATION CHARACTERIZATION AND FUNCTIONAL PROPERTIES Rafika Bibi1 2 Hind Mokrane1 Khaled Khaladi1 2 Houria Amoura1 Address es Prof. Hind Mokrane PhD. 1 Laboratoire des produits bioactifs et de valorisation de la biomasse Ecole Normale Supérieure . 92 16050 Vieux-Kouba Algiers Algeria phone number 00213555253835. 2 Département des sciences de la matière Université Alger 1 Benyoucef Benkhedda Algiers Algeria. Corresponding author or mokraneh2017@ https ARTICLE INFO ABSTRACT Received 5. 9. 2020 Pearl millet is a gluten free cereal resistant to drought diseases and pests. The major protein fraction in pearl millet is prolamin the Revised 18. 5. 2021 alcohol soluble fraction called pennisetin. Researches on the factors affecting pennisetin extraction and on its functional properties are Accepted 28. 5. 2021 still very scant. In this paper the effect of temperature reducing agents β-mercaptoethanol dithiothreitol or sodium metabisulfite and Published 1. 10. 2021 sodium hydroxide on pennisetin extraction was assessed. Samples were characterized by protein and amino acid AA analysis SDS- Page and reversed phase high-performance liquid chromatography RP-HPLC . Pennisetin was extracted with high protein purity from pearl millet grain flour PMF using 70 aqueous ethanol containing 1 sodium metabisulfite and sodium Regular article hydroxide at 60 C. SDS-Page confirmed pennisetin extraction and showed three subunits corresponding to the 27- 22- and 12 kDa- pennisetins. The percentage of essential amino acids in pennisetin was higher than that in PMF . The hydrophobic character of pennisetin was confirmed by the presence of of hydrophobic amino acids. Functional properties of pennisetin and PMF were investigated. Compared to PMF pennisetin exhibited comparable oil and water binding .

• Sinh học
• Biotechnology and food sciences
• Pearl millet
• Pennisetum glaucum
• Pennisetin extraction
• RP HPLC
• Functional properties
• Bacteriophages target
• Food borne pathogens in food industry
• Food borne illnesses
• Bacterial pathogens
• Design of novel pyrimidine analogs
• Synthesis of novel pyrimidine analogs
• Anti tubercular agents targeting thymidine kinase domain
• Molecular docking
• Thymidine kinase
• Nutritional factors influencing the biosynthesis
• Amyloid bioemulsifier beam1 applicable in food industry
• Plackett Burman
• Response surface method
• Solibacillus silvestris AM1 S silvestris
• Serotyping of salmonella isolates
• Salmonella isolates from food matrices
• Clinical specimens from Lebanon
• Multidrug resistant bacteria
• Foodborne diseases
• Maillard reaction marker
• Foods including jam
• Baby food
• Formation of chosen MRPs
• Nutritional content of souro
• Iranian traditional food
• In vitro evaluation
• Bile salt resistance
• Bacterial diversity in raw fish ceviche
• Raw fish ceviche
• Food safety
• The analyzed samples
• Chitosan nanoparticles
• Nanoparticles conjugate bacteriocin
• Lactococcus lactis subsp lactis
• Food borne pathogenic bacteria
• Magnetic micro dispersive solid phase extraction
• Food additive
• The FDA guidelines
• Self assembling manner
• Salt reduction
• Sensory preference
• Physicochemical parameters
• Image analysis
• External preference mapping
• Antitumor properties of vegetable oil extract
• Green microalga coelastrella sp
• Vegetable oil extract
• Antitumor activity
• Sunflower oil extract
• Taxonomic investigation of euplanktonic diatom communities
• New record
• High copper exposed area
• Harmful species Halamphora coffeiformis
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• Phylogenetic groups
• Pathogenicity islands PAIs
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• Chemical characteristics of different cassava varieties
• The quality attributes
• Cassava varieties
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• Dihydroquercetin on functional parameters
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• Bioconversion of wine pomace by lentinus edodes
• Solid state system
• Lentinus edodes
• Phenolic compounds
• High polymer DNA
• Sspecies of hydrobionts
• Bovine spleen 4 11 times
• Degree of depolymerization
• Gray mold disease
• Visible/near infrared
• Early detection
• Multivariate data analysis
• Principal component analysis
• Usnea longissima
• Infectious disease
• Chromobacterium violaceum
• Evaluate antimicrobial
• Antiquorum sensing
• Locally available preservatives
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• Seasonal variations
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• Open waters
• Fatty acid composition
• High PUFA content
• Burkholderia cepacia bam 12
• Mungbean field in Rajasthan augments plant growth
• Agricultural field soil of Gujarat
• Burkholderia cepacia catecholate
• Phosphate solubilizing bacteria
• Novel association between GnRHR gene
• Growth traits in awassi
• Single strand conformation polymorphism
• GnRHR gene exon 2
• Bio profiling
• Antimycobacterial bacteriocin
• Bacillus subtilis of ethnomedicinal origin
• Bioassay guided TLC techniques
• Bacteriocin producing lactic acid bacteria
• Salted dried camel meat
• Goat cheese against staphylococcus aureus
• Staphylococcus aureus