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The major protein fraction in pearl millet is prolamin the alcohol soluble fraction called pennisetin. Researches on the factors affecting pennisetin extraction and on its functional properties are still very scant. In this paper, the effect of temperature, reducing agents (β-mercaptoethanol, dithiothreitol or sodium metabisulfite) and sodium hydroxide on pennisetin extraction was assessed. | IMPROVEMENT OF PEARL MILLET Pennisetum glaucum L. R. BR PROLAMIN EXTRACTABILITY CHROMATOGRAPHIC SEPARATION CHARACTERIZATION AND FUNCTIONAL PROPERTIES Rafika Bibi1 2 Hind Mokrane1 Khaled Khaladi1 2 Houria Amoura1 Address es Prof. Hind Mokrane PhD. 1 Laboratoire des produits bioactifs et de valorisation de la biomasse Ecole Normale Supérieure B.P. 92 16050 Vieux-Kouba Algiers Algeria phone number 00213555253835. 2 Département des sciences de la matière Université Alger 1 Benyoucef Benkhedda Algiers Algeria. Corresponding author hind.mokrane@g.ens-kouba.dz or mokraneh2017@gmail.com https doi.org 10.15414 jmbfs.3674 ARTICLE INFO ABSTRACT Received 5. 9. 2020 Pearl millet is a gluten free cereal resistant to drought diseases and pests. The major protein fraction in pearl millet is prolamin the Revised 18. 5. 2021 alcohol soluble fraction called pennisetin. Researches on the factors affecting pennisetin extraction and on its functional properties are Accepted 28. 5. 2021 still very scant. In this paper the effect of temperature reducing agents β-mercaptoethanol dithiothreitol or sodium metabisulfite and Published 1. 10. 2021 sodium hydroxide on pennisetin extraction was assessed. Samples were characterized by protein and amino acid AA analysis SDS- Page and reversed phase high-performance liquid chromatography RP-HPLC . Pennisetin was extracted with high protein purity 90.1 from pearl millet grain flour PMF using 70 aqueous ethanol containing 1 sodium metabisulfite and 0.2 sodium Regular article hydroxide at 60 C. SDS-Page confirmed pennisetin extraction and showed three subunits corresponding to the 27- 22- and 12 kDa- pennisetins. The percentage of essential amino acids in pennisetin 68.55 was higher than that in PMF 41.64 . The hydrophobic character of pennisetin was confirmed by the presence of 45.49 of hydrophobic amino acids. Functional properties of pennisetin and PMF were investigated. Compared to PMF pennisetin exhibited comparable oil and water binding .
