TAILIEUCHUNG - Molecular investigation of Rifampicin resistance in clinical isolates of Mycobacterium ulcerans in Côte D'ivoire

M. ulcerans is the etiologic agent of Buruli ulcer. The clinical diagnosis of infection with this mycobacterium is based on microbiological analyzes, including PCR, which uses the nucleotide sequences of the primers to detect the targeted sequences. Cases of rifampicin resistance were recorded in mice experimentally infected with M. ulcerans strains and treated with rifampicin in previous studies. In this study, clinical isolates were confirmed as M. ulcerans strains. These isolates are from samples of patients with Buruli ulcer. The PCR gave a positivity rate of for the detection of the IS 2404 sequence and made it possible to highlight the virulence gene in most of the strains studied with a positivity rate of for the detection of the sequence IS-KR. However, the RpoB gene could not be found in any of the strains. Thus it gives a positivity rate of 0% for the detection of this gene. The results show that most strains of M. ulcerans secrete mycolactone. The production of this toxin is the consequence of a mutation in the IS-KR gene. They also show that rifampicin has an effective bactericidal activity against M. ulcerans strains and that resistance to this antibiotic results from a mutation of the RpoB gene. | Molecular investigation of Rifampicin resistance in clinical isolates of Mycobacterium ulcerans in Côte D'ivoire

• Sinh học
• Buruli ulcer
• Ivory coast
• RpoB gene
• ISKR sequence
• Mycobacterium ulcerans
• Clinical isolates
• Speciation and characterization
• Candida species in clinical isolates
• Predisposing conditions for candidiasis
• Various clinical samples
• Antibiotic susceptibility
• Neonatal septicemia
• Clinical isolates of neonatal septicemia
• Antibiotic susceptibility pattern
• Pseudomonas aeruginosa
• Antibiotic resistance
• Beta lactamase
• Producing clinical isolates
• Biofilm detection
• Prevalence of MRSA
• MRSA and biofilm associated gene
• Biofilm associated gene
• Clinical isolates of staphylococci
• Investigation of antibiofilm potential
• Antibiofilm potential
• Argemone Mexicana
• Calotropis Gigantea against clinical isolates
• Against clinical isolates
• Panton valentine leukocidin gene
• Staphylococcus aureus
• Rapid detection
• Staphylococcus aureus clinical isolates
• Clinical isolates of staphylococcus aureus
• Tertiary care hospital
• Prevalence of MRSA among clinical isolates
• Candida isolates
• Speciation of candida isolates
• Clinical samples
• Conventional and chromagar method
• Chromagar method
• Bovine mastitis
• Bovine clinical mastitis
• Antibiogram of the isolates
• Isolates in Odisha
• Morphological and biochemical characteristics
• Biotechnology and food sciences
• Serotyping of salmonella isolates
• Salmonella isolates from food matrices
• Clinical specimens from Lebanon
• Multidrug resistant bacteria
• Foodborne diseases
• Antifungal susceptibility
• Antifungal susceptibility testing
• Candida isolates in various clinical samples
• Normal flora of human beings
• Phenotypic detection
• Carbapenem resistance
• Escherichia coli
• Klebsiella isolates obtained
• OprD protein profile
• Pseudomonas Aeruginosa isolates resistant
• Imipenem from patients
• Staphylococcal isolates and Pathogens
• Coagulase negative staphylococcal isolates
• Clinically significant specimens
• Resistance of these organisms
• Clinical specimens
• Sub clinical mastitis
• Antibiogram of bacterial isolates
• Bacterial isolates
• Bubaline subclinical mastitis
• Identification of bacterial pathogens
• Dexamethasone abrogates
• Antibiofilm activities
• Different drugs against clinical isolates
• Inducible resistance
• Detection of inducible clindamycin resistance
• Erythromycin in clinical isolates
• Prevalence among methicillin resistant
• Stenotrophomonas maltophilia
• Drug resistance
• Co trimoxazole resistance
• Susceptibility to co trimoxazole
• Honey lemon juice mixtures
• In vitro
• Antimicrobial activity
• Bamenda Cameroon
• Respiratory tract infections
• Vancomycin resistant Enterococci
• Entero cocci fecalis
• Various clinical sample isolates
• Multidrug resistance exhibited
• Serious life threatening VRE infections
• Piperacillin Tazobactum
• Detection and characterization
• Extended spectrum β lactamases
• Salmonella isolates of meat
• Milk and human clinical samples
• Different districts of Chhattisgarh
• Amp C
• Producing enterobacteriaceae isolates
• Extended spectrum beta lactamse
• Multidrug resistance organisms
• Phenotypic detection of MRSA
• Inducible clindamycin resistance
• Staphylococcus study done
• D test positive
• Klebsiella pneumoniae
• ESBL producing Klebsiella pneumoniae
• Antimicrobial susceptibility pattern
• ESBL producing clinical isolates
• Appropriate infection control measures