TAILIEUCHUNG - A comparative study of EGF effects on in vitro bovine embryo development in monoculture and sequential media

Different culture system conditions can affect cleavage, zygote genome activation, embryo development, and viability. The purpose of the present study was to compare a monoculture synthetic oviductal fluid medium with modified sequential G1/G2 media and also to investigate the effect of epidermal growth factor (EGF) on bovine embryo development. | Turkish Journal of Biology Turk J Biol (2013) 37: 670-674 © TÜBİTAK doi: Research Article A comparative study of EGF effects on in vitro bovine embryo development in monoculture and sequential media 1, 2, 3, Azadeh ZAHMATKESH *, Mahmood AMIRI ROUDBAR *, Morteza DALIRI JOUPARI ** Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran 2 Department of Animal Sciences, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran 3 Department of Animal and Marine Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran 1 Received: Accepted: Published Online: Printed: Abstract: Different culture system conditions can affect cleavage, zygote genome activation, embryo development, and viability. The purpose of the present study was to compare a monoculture synthetic oviductal fluid medium with modified sequential G1/G2 media and also to investigate the effect of epidermal growth factor (EGF) on bovine embryo development. Oocytes were aspirated from 2- to 6-mm ovarian follicles and matured in maturation media. After in vitro fertilization of matured oocytes, presumptive zygotes were cultured in test media with or without additional exogenous EGF. Results indicated that EGF significantly increased blastocyst formation but not cleavage rate in both media. There was no significant difference between the sequential and the monoculture media. Key words: Blastocyst, cleavage, culture system, epidermal growth factor 1. Introduction Development of bovine embryo culture systems is very important for producing viable embryos for transfer as well as for promoting scientific research (Kim et al., 1993). The quality and survival of in vitro-produced bovine embryos has not reached the maximal rate of in vivo-derived embryos (Boni et al., 1999). In recent decades, different systems have

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