TAILIEUCHUNG - Mucus of Achatina fulica stimulates mineralization and inflammatory response in dental pulp cells

Dental pulp tissue contains stem cells that can be isolated and used for regenerative medicine in tooth restoration or autologous transplantation. The aim of this study was to observe the mineralization and gene expression in dental pulp cells (DPCs) following treatment with snail mucus. | Turkish Journal of Biology Turk J Biol (2016) 40: 353-359 © TÜBİTAK doi: Research Article Mucus of Achatina fulica stimulates mineralization and inflammatory response in dental pulp cells 1, 1 1 1 1 Fahsai KANTAWONG *, Pichaporn THAWEENAN , Sutinee MUNGKALA , Sawinee TAMANG , Ruthairat MANAPHAN , 2 3 4 Phenphichar WANACHANTARARAK , Teerasak E-KOBON , Pramote CHUMNANPUEN 1 Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 2 The Dental Research Center, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand 3 Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand 4 Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand Received: Accepted/Published Online: Final Version: Abstract: Dental pulp tissue contains stem cells that can be isolated and used for regenerative medicine in tooth restoration or autologous transplantation. The aim of this study was to observe the mineralization and gene expression in dental pulp cells (DPCs) following treatment with snail mucus. Snail mucus was collected from adult Achatina fulica and processed as a dry powder by the freeze-drying technique. The mucus powder was dissolved in a culture medium at a concentration of 15 µg/mL. DPCs were prepared by the outgrowth technique and cultured in a 6-well plate at a density of 5 × 104 cells per well. A mucus-supplemented medium (15 µg/mL) was added to each well. Cell culture was maintained for 3 weeks. The results of Alizarin Red S staining indicated that the DPCs cultured in a medium supplemented with snail mucus showed a higher number of mineralized nodules as compared with the control group cultured in a normal medium. The increased expression of osteopontin and NF-κB reflected the differentiation process of DPCs into bone cells. Snail mucus also induced the

• Sinh học
• Dental pulp cells
• Snail mucus
• Achatina fulica
• Inflammatory genes
• Human dental pulp cells
• Dental pulp
• Mesenchymal stem cells
• Definitive endoderm
• Cryopreserved dental pulp tissues
• Hepatocyte like cells
• Embryonic stem cells
• Neural crest
• Human dental pulp
• Cell based therapies
• Postnatal dental pulp stem cells
• Cell proliferation
• Stemness maintenance
• BMC Molecular and Cell Biology
• Stem cells
• Cumulative population doublings
• Cellular senescence
• Multi potency
• Genetic stability
• Osteogenic differentiation
• Cell culture models
• Regenerative medicine
• Bone regeneration
• Dental pulp stem cells
• Human periapical cysts mesenchymal stem cells
• hPCy MSCs
• Human dental pulp stem cells
• Human umbilical cord mesenchymal stem cells
• Non contact coculture system
• Enhanced osteogenic genes
• MTOR signaling pathway
• BMC Veterinary Research
• Cell therapy
• Umbilical cord