TAILIEUCHUNG - Monitoring of germin synthesis by pre–absorbed anti–germin serum

The transition of a quiescent plant embryo into a vigorously growing embryo revealed some nascent synthesis of gene products, among them germin has been characterized. Germin is a water soluble homopentameric glycoprotein whose synthesis and accumulation appear to be closely related to early wheat embryo development. | Turk J Biol 24 (2000) 271–278 © TÜBİTAK Monitoring of Germin Synthesis by Pre–Absorbed Anti–Germin Serum Mahmut ÇALIŞKAN and Andrew C. CUMING Mustafa Kemal University, Department of Biology, 31040 Hatay–TURKEY Received: Abstract: The transition of a quiescent plant embryo into a vigorously growing embryo revealed some nascent synthesis of gene products, among them germin has been characterized. Germin is a water soluble homopentameric glycoprotein whose synthesis and accumulation appear to be closely related to early wheat embryo development. Germin synthesis was monitored by employing an anti–germin serum which had been pre–adsorbed with wheat extract and optimized in order to decrease nonspecific binding. Germin accumulation was shown to occur in germinating embryos from 16h up to 6 day post–imbibition. Germin as a rare protein in wheat seedlings was detected as a dark band on X–rays in amounts as small as 20 µl protein extracts. Neither germin in mature wheat plant organs nor germinated embryos were detected prior to 16 hours of imbibition. Key Words: Early Wheat development, Germin, Western blotting. Germin Sentezinin Spesiyalitesi Artırılmış Germin Antikorları ile İzlenmesi Özet: Bitki embriyoları dormant halden aktif bir büyüme dönemine girdikleri zaman, bazı yeni gen ürünleri sentezlerler. Bu ürünlerden germin proteini karakterize edilmiştir. Germin, suda çözünen, homopentamerik bir glikoproteindir. GermiN sentezi ve birikimi buğday embriyosunun ilk büyüme evreleri ile yakından ilgilidir. Germin sentezi spesifik olmayan bağlanmaları önlemek için buğday–ekstraktı ile on muamele edilmiş anti–germin antikoru kullanılarak analiz edilmiştir. Germin proteini 16 saat ile 6 gün arası tüm filizlenen buğday embriyolarında bulunmuştur. Germin az bulunan bir protein olmasına rağmen, 20 µL protein ekstraktında bile X–Ray filmi üzerinde koyu bir band olarak antikorlar tarafından belirlenmiştir. Germin olgun buğday bitkisi organlarında ve 16 saatten önceki .

• Sinh học
• Early Wheat development
• Western blotting
• Homopentameric glycoprotein
• Germin synthesis
• Wheat embryo
• Early wheat embryo development
• Germin protein
• Anti germin sera
• Gel blot analysis
• BMC Plant Biology
• RNA Seq
• Grain development
• Post anthesis
• Cell wall
• Wheat seeds
• Differentially expressed protein
• Seed germination
• Plant tissue culture
• Wheat genetic
• Plant regeneration
• Established in soil
• Somatic embryogenesis
• Gold coating
• Biolistic transformation
• Immature embryo
• Single copy events
• Transformation frequency
• Triticum aestivum
• BMC Genetics
• RNA sequencing
• Immature embryo culture
• Phenylpropanoid biosynthesis
• Plant hormone signaling
• Mite pests
• Stored grains
• Germination rates
• Crude aqueous extracts