TAILIEUCHUNG - Kinetic studies with crude tomato alcohol dehydrogenase

Tomato alcohol dehydrogenase (ADH) is very important in the formation of fresh tomato aroma volatiles. The kinetic characterization and isolation of this enzyme may promote some in vitro applications. | Turk J Agric For 26 (2002) 141-145 © TÜB‹TAK Kinetic Studies with Crude Tomato Alcohol Dehydrogenase Emin YILMAZ Çanakkale Onsekiz Mart University, Faculty of Engineering-Architecture Department of Food Engineering, 17100, Çanakkale - TURKEY Received: Abstract: Tomato alcohol dehydrogenase (ADH) is very important in the formation of fresh tomato aroma volatiles. The kinetic characterization and isolation of this enzyme may promote some in vitro applications. Kinetic studies of crude tomato ADH associated with NAD+ cofactor revealed Km values of mM for ethanol and mM for NAD+, a Vmax value of µM/min, and an activation energy of kcal/mole. Variable pH measurements indicated that the enzyme is optimally active in a narrow range above pH . Dalziel coefficients were consistent with a sequential but not ordered mechanism. Also the enzyme was found to be heat labile. Crude tomato alchol dehydrogenase can be a suitable enzyme for some biotechnological applications. Key Words: tomato, alcohol dehydrogenase, Dalziel coefficients, enzyme kinetics Ham Özüt Domates Alkol Dehidrojenaz›yla Kinetik Çal›flmalar Özet: Domates alkol dehidrojenaz› (ADH), taze domates uçucu aromatiklerinin oluflumunda çok önemlidir. Bu enzimin kinetik karakterizasyonu ve izolasyonu baz› in vitro uygulamalara önayak olabilir. NAD+ kofaktörü gerektiren ham özüt domates alkol dehidrojenaz› ile yap›lan kinetik çal›flmalar sonunda etanol için 2,03 mM ve NAD+ için 0,064 mM Km de¤erleri ile 24,51 µM/min Vmax de¤eri, ve 0,0208 kcal/mol aktivasyon enerjisi de¤eri bulunmufltur. Çeflitli pH ölçümleri sonunda enzimin pH ’in üzerindeki dar bir aral›kta optimum aktiviteye sahip oldu¤u belirlenmifltir. Dalziel katsay›lar› düzenli s›ral› de¤il sadece s›ral› mekanizma ile uyumludur. Ayr›ca enzimin s›cakl›¤a karfl› hassas oldu¤u bulunmufltur. Ham özüt domates alkol dehidrojenaz› baz› biyoteknoloji uygulamalar› için uygun bir enzim olabilir. Anahtar Sözcükler: domates, alkol .

• Nông nghiệp
• Tomato alcohol dehydrogenase
• Alcohol dehydrogenase
• Dalziel coefficients
• Enzyme kinetics
• Kinetic characterization
• Oxylipin pathway
• Hydroperoxide lyase
• Tomato volatiles
• Alcohol compounds
• Journal of Chemistry
• Bifunctionalized SBA15
• Metal adsorption
• Kinetic model
• Adsorption isotherm
• Mesoporous material
• BMC Plant Biology
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