TAILIEUCHUNG - Lecture Principles of biochemistry - Chapter 19 (part 2):

After completing this chapter, students will be able to draw the structure of short DNA and RNA molecules (3 to 4 nucleotides long), structure of each nucleotide must be correctly drawn, as should the phosphodiester linkage between each nucleotide; draw all nucleotides in the anti conformation; know which side is the 5’ end and which side is the 3’end; understand what is base pairing. Know how many H-bonds form between which bases;. | Chapter 19 (part 2) Nucleic Acids DNA 1o Structure - Linear array of nucleotides 2o Structure – double helix 3o Structure - Super-coiling, stem-loop formation 4o Structure – Packaging into chromatin Determination of the DNA 1o Structure (DNA Sequencing) Can determine the sequence of DNA base pairs in any DNA molecule Chain-termination method developed by Sanger Involves in vitro replication of target DNA Technology led to the sequencing of the human genome DNA Replication DNA is a double-helical molecule Each strand of the helix must be copied in complementary fashion by DNA polymerase Each strand is a template for copying DNA polymerase requires template and primer Primer: an oligonucleotide that pairs with the end of the template molecule to form dsDNA DNA polymerases add nucleotides in 5'-3' direction Chain Termination Method Based on DNA polymerase reaction 4 separate rxns Each reaction mixture contains dATP, dGTP, dCTP and dTTP Each reaction also contains a small amount of one .

• Sinh học
• Principles of biochemistry
• Lecture Principles of biochemistry
• Nguyên lý hóa sinh
• Biochemical compounds
• Nucleic acids
• DNA Replication
• Ebook Principles of biochemistry
• Principles of biochemistry (4th edition)
• The glyoxylate cycle
• Fatty acid catabolism
• Electron Transfer reactions
• Evolutionary foundations
• Protein interactions modulated
• Nucleic acid structure
• Biological systems
• Biological macromolecules
• Chemical principles
• Properties of water
• Hydrogen bonding
• Amino acids
• Amino acid structure
• Protein purification
• Cell extract
• Protein classification
• Protein 3 Dimensional structure
• Protein folding
• Protein 3 D structure
• 3 D structure
• Oxygen binding curve
• Enzyme kinetics
• Rate constants
• Catalytic Power
• Classes of enzymes
• Inhibitor kinetics
• Enzyme regulation
• Allosteric regulation
• Enzyme action
• Mechanisms of enzyme action
• Ions Cofactors
• Metal ion
• Biotin cofactor
• Conformation of monosaccharides
• Carbohydrate classifications
• Oligo carbohydrate
• Polysaccharides carbohydrate
• Lipid subclasses
• Acyl lipids
• Fluid mosaic model
• membrane transporters
• Metabolism proceed
• Anaeorbic process
• Triose Phosphate Isomerase
• Regulation of glycolysis
• Control points in glycolysis
• Citric acid cycle
• TCA cycle