TAILIEUCHUNG - Production, purification, characterization, and applications of lipases

In addition to the aforementioned direct benefits of using alternative fuels for cement manufacturing, there are numerous life-cycle benefits and avoided costs that are realized. Alternative fuels are essentially the waste products of other industrial or agricultural processes, and due to their sheer volume and potentially their toxicity, they pose a major solid waste management challenge in many countries. Thermal combustion of these materials is a way to both capture their embodied energy and significantly reduce their volumes; this can be done in dedicated waste-to-energy incinerators or at cement plants. . | ELSEVIER Biotechnology Advances 19 2001 627-662 Biotechnology Advances Research review paper Production purification characterization and applications of lipases Rohit Sharmaa Yusuf Chistib Uttam Chand Banerjee3 National Institute of Pharmaceutical Education and Research Sector 67 SAS Nagar Mohali Punjab 160062 India bInstitute of Technology and Engineering Massey University Private Bag 11 222 Palmerston North New Zealand Abstract Lipases triacylglycerol acylhydrolases EC catalyze the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. Lipases occur widely in nature but only microbial lipases are commercially significant. The many applications of lipases include speciality organic syntheses hydrolysis of fats and oils modification of fats flavor enhancement in food processing resolution of racemic mixtures and chemical analyses. This article discusses the production recovery and use of microbial lipases. Issues of enzyme kinetics thermostability and bioactivity are addressed. Production of recombinant lipases is detailed. Immobilized preparations of lipases are discussed. In view of the increasing understanding of lipases and their many applications in high-value syntheses and as bulk enzymes these enzymes are having an increasing impact on bioprocessing. 2001 Elsevier Science Inc. All rights reserved. Keywords Esters Enzymes Esterases Lipases 1. Introduction The use of enzyme-mediated processes can be traced to ancient civilizations. Today nearly 4000 enzymes are known and of these about 200 are in commercial use. The majority of the industrial enzymes are of microbial origin. Until the 1960s the total sales of enzymes were Corresponding author. Tel. 91-172-214682 fax 91-172-214692. E-mail address niper@ . Banerjee . 0734-9750 01 - see front matter 2001 Elsevier Science Inc. All rights reserved. PII S0734-9750 01 00086-6 628 R. Sharma et al. Biotechnology Advances 19 2001 627-662 only a few million dollars .

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• Aerobic Digestion
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• olid State
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• Pozzolanic Materials