TAILIEUCHUNG - Báo cáo Y học: Characterization of a novel silkworm (Bombyx mori ) phenol UDP-glucosyltransferase

Sugar conjugation is a major pathway for the inactivation and excretion of both endogenous and exogenous compounds. We report here the molecular cloning and functional characterization of a phenol UDP-glucosyltransferase (UGT) from the silkworm, Bombyx mori, which was named BmUGT1. The complete cDNA clone is kb, and the gene is expressed in several tissues of fifth-instar larvae, including fat body, midgut, integument, testis, silk gland and haemocytes. The predicted protein comprises 520 amino acids and has 30% overall amino-acid identity with other members of the UGT family. The most conserved region of the protein is the C-terminal half,. | Eur. J. Biochem. 269 819-825 2002 FEBS 2002 Characterization of a novel silkworm Bombyxmori phenol UDP-glucosyltransferase Teresa Luque1 Kazuhiro Okano2 and David R. O Reilly1 1 Department of Biology Imperial College of Science Technology and Medicine London SW7 2AZ UK Laboratory of Molecular Entomology and Baculovirology Riken Wako Japan Sugar conjugation is a major pathway for the inactivation and excretion of both endogenous and exogenous compounds. We report here the molecular cloning and functional characterization of a phenol UDP-glucosyltransferase UGT from the silkworm Bombyx mori which was named BmUGTl. The complete cDNA clone is kb and the gene is expressed in several tissues of fifth-instar larvae including fat body midgut integument testis silk gland and haemocytes. The predicted protein comprises 520 amino acids and has w 30 overall amino-acid identity with other members of the UGT family. The most conserved region of the protein is the C-terminal half which has been implicated in binding the UDP-sugar. BmUGT1 was expressed in insect cells using the baculovirus expression system and a range of compounds belonging to diverse chemical groups were assessed as potential substrates for the enzyme. The expressed enzyme had a wide substrate specificity showing activity with flavonoids coumarins terpenoids and simple phenols. These results support a role for the enzyme in detoxication processes such as minimizing the harmful effects of ingested plant allelochemicals. This work represents the first instance where an insect ugt gene has been associated with a specific enzyme activity. Keywords Bombyx mori detoxication insect UDP-glycosyltransferase. The UDP-glycosyltransferases UGTs are a superfamily of enzymes that play a central role in the detoxication and elimination of a wide range of endogenous and exogenous compounds. Members of this superfamily are present in animals plants bacteria and viruses suggesting an ancient origin reviewed in 1-3 . These .

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