TAILIEUCHUNG - Báo cáo Y học: Induction of chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI ) gene expression is mediated by ETS factor binding sites

Chickenovalbuminupstreampromoter-transcriptionfactorI (COUP-TFI, or NR2F1) is an orphan nuclear receptor that plays amajor role in the development of the nervous system. We show here that three ETS response elements in the COUP-TFIpromoter mediate its transcription. A reporter gene containing theseETSbinding sites is activatedbyEts-1, while the same reporter with point mutations on all three ETS response elements is not. | Eur. J. Biochem. 269 317-325 2002 FEBS 2002 Induction of chicken ovalbumin upstream promoter-transcription factor I COUP-TFI gene expression is mediated by ETS factor binding sites Ramiro Salas Fabrice G. Petit Carlos Pipaon f Ming-Jer Tsai and Sophia Y. Tsai Department of Molecular and Cellular Biology Baylor College of Medicine Houston TX USA Chicken ovalbumin upstream promoter-transcriptionfactor I COUP-TFI or NR2F1 is an orphan nuclear receptor that plays a major role in the development of the nervous system. We show here that three ETS response elements in the COUP-TFI promoter mediate its transcription. A reporter gene containing these ETS binding sites is activated by Ets-1 while the same reporter with point mutations on all three ETS response elements is not. We also show that Ets-1 binds to these response elements and that other ETS factors also transactivate the COUP-TFIpromoter. In addition COUP-TFI is coexpressed with some ETS factors in the mouse embryo. These results indicate that members of the ETS family can activate COUP-TFI gene expression. Keywords COUP-TFI ETS gene expression transcription orphan receptors. Chicken ovalbumin upstream promoter-transcription factors COUP-TFs are among the best characterized orphan members of the nuclear receptor superfamily 1 . COUP-TFs have been shown to be negative regulators of the transcription of many genes 1 2 but can also act as activators of gene transcription 1 3 . In mammals two COUP-TF genes have been identified COUP-TFI and COUP-TFII. Although they have different physiological functions 4 5 sequence analysis and molecular studies indicate that they share similar properties. The expression patterns of COUP-TFI and COUP-TFII have been extensively studied in a number of species 6 . In the mouse COUP-TFI is first detected at the embryonic day its expression reaches a peak at declines before birth 6 and remains low during adulthood. COUP-TFI null mice die perinatally and exhibit neuronal .

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