TAILIEUCHUNG - Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens

The complete coding sequence of a BDIX rat gene homo-logous to the human ABO gene was determined. Identifi-cation of the exon–intron boundaries, obtained by comparison of the coding sequence with rat genomic sequences from data banks, revealed that the rat gene structure is identical to that of the human ABO gene. It localizes to rat chromosome 3 (q11-q12), a region homolo-gous to human 9q34. | Eur. J. Biochem. 269 4040-4047 2002 FEBS 2002 doi Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens Anne Cailleau-Thomas1 Beatrice Le Moullac-Vaidye1 Jezabel Rocher 1 Daniele Bouhours2 Claude Szpirer3 and Jacques Le Pendu1 1INSERM U419 Institut de Biologie Nantes France 2INSERM U539 Faculte de Medecine Nantes Cedex France 3IBMM Universite Libre de Bruxelles Gosselies Belgium The complete coding sequence of a BDIX rat gene homologous to the human ABO gene was determined. Identification of the exon-intron boundaries obtained by comparison of the coding sequence with rat genomic sequences from data banks revealed that the rat gene structure is identical to that of the human ABO gene. It localizes to rat chromosome 3 q11-q12 a region homologous to human 9q34. Phylogenetic analysis of a set of sequences available for the various members of the same gene family confirmed that the rat sequence belongs to the ABO gene cluster. The cDNA was transfected in CHO cells already stably transfected with an a1 2fucosyltransferase in order to express H oligosaccharide acceptors. Analysis of the transfectants by flow cytometry indicated that A but not B epitopes were synthesized. Direct assay of the enzyme activity using 2 fucosyllactose as acceptor confirmed the strong UDP-GalNAc Fuca1 2GalaGalNAc transferase A transferase activity of the enzyme product and allowed detection of a small UDP-Gal Fuca1 2GalaGal transferase B transferase activity. The presence of the mRNA and of the A and B antigens was searched in various BDIX rat tissues. There was a general good concordance between the presence of the mRNA and that of the A antigen. Tissue distributions of the A and B antigens in the homozygous BDIX rat strain were largely different indicating that these antigens cannot be synthesized by alleles of the same gene in this rat inbred strain. Keywords ABO .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medical knowledge
• cytoplasm
• analysis of cytoplasmic
• medical research
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine