TAILIEUCHUNG - Báo cáo khoa học: Purification and gene cloning ofFundulus heteroclitus hatching enzyme A hatching enzyme system composed of high choriolytic enzyme and low choriolytic enzyme is conserved between two different teleosts,Fundulus heteroclitusand medaka Oryzias latipes

Two cDNA homologues of medaka hatching enzyme)high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE) – were cloned from Fundulus heteroclitusembryos. Amino acid sequences of the mature forms ofFundulusHCE (FHCE) and LCE (FLCE) were and iden-tical to those of medaka HCE and LCE, respectively. In addition, phylo-genetic analysis clearly showed that FHCE and FLCE belonged to the clades of HCE and LCE, respectively. | ềFEBS Journal Purification and gene cloning of Fundulus heteroclitus hatching enzyme A hatching enzyme system composed of high choriolytic enzyme and low choriolytic enzyme is conserved between two different teleosts Fundulus heteroclitus and medaka Oryzias latipes Mari Kawaguchi1 Shigeki Yasumasu1 Akio Shimizu2 Junya Hiroi3 Norio Yoshizaki4 Koji Nagata5 11 Masaru Tanokura5 and Ichiro Iuchi1 1 Life Science Institute Sophia University Tokyo Japan 2 NationalResearch Institute of Fisheries Science Fisheries Research Agency Kanazawa Japan 3 Department of Anatomy St. Marianna University Schoolof Medicine Kawasaki Japan 4 Department of BiologicalDiversity Faculty of Agriculture Gifu University Japan 5 Department of Applied BiologicalChemistry Graduate Schoolof Agriculturaland Life Sciences The University of Tokyo Japan Keywords Fundulus heteroclitus hatching enzyme astacin protease family intron-less gene Correspondence I. Iuchi Life Science Institute Sophia University 7-1 Kioi-cho Chiyoda-ku Tokyo Japan 102-8554 Fax 81 3 3238 3393 Tel 81 3 3238 3393 E-mail i-iuchi@ Note The nucleotide sequence data reported in the present paper will appear in the DDBJ EMBL GenBank nucleotide sequence databases with accession number AB210813 and AB210814 Received 27 April2005 revised 28 June 2005 accepted 4 July 2005 doi Two cDNA homologues of medaka hatching enzyme - high choriolytic enzyme HCE and low choriolytic enzyme LCE - were cloned from Fundulus heteroclitus embryos. Amino acid sequences of the mature forms of Fundulus HCE FHCE and LCE FLCE were and identical to those of medaka HCE and LCE respectively. In addition phylogenetic analysis clearly showed that FHCE and FLCE belonged to the clades of HCE and LCE respectively. Exon-intron structures of FHCE and FLCE genes were similar to those of medaka HCE intronless and LCE 8-exon-7-intron genes respectively. Northern blotting and wholemount in situ hybridization .

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