TAILIEUCHUNG - Báo cáo khoa học: A novel tachykinin-related peptide receptor of Octopus vulgaris – evolutionary aspects of invertebrate tachykinin and tachykinin-related peptide

The tachykinin (TK) and tachykinin-related peptide (TKRP) family repre-sent one of the largest peptide families in the animal kingdom and exert their actions via a subfamily of structurally related G-protein-coupled receptors. In this study, we have identified a novel TKRP receptor from the Octopus heart, oct-TKRPR. | ỊFEBS Journal A novel tachykinin-related peptide receptor of Octopus vulgaris - evolutionary aspects of invertebrate tachykinin and tachykinin-related peptide Atsuhiro Kanda Kyoko Takuwa-Kuroda Masato Aoyama and Honoo Satake Suntory Institute for Bioorganic Research Osaka Japan Keywords evolution Octopus vulgaris oct-TKRPR tachykinin-related peptide receptor tachykinin Correspondence A. Kanda Suntory Institute for Bioorganic Research 1-1-1 Wakayamadai Shimamoto-cho Mishima-gun Osaka 618-8503 Japan Fax 81 75 962 2115 Tel 81 75 962 3743 E-mail kanda@ Database Nucleotide sequence data are available in the DDBJ EMBL GenBank databases under the accession number AB096700 Received 16 December 2006 revised 17 February 2007 accepted 28 February 2007 doi The tachykinin TK and tachykinin-related peptide TKRP family represent one of the largest peptide families in the animal kingdom and exert their actions via a subfamily of structurally related G-protein-coupled receptors. In this study we have identified a novel TKRP receptor from the Octopus heart oct-TKRPR. oct-TKRPR includes domains and motifs typical of G-protein-coupled receptors. Xenopus oocytes that expressed oct-TKRPR like TK and TKRP receptors elicited an induction of membrane chloride currents coupled to the inositol phosphate calcium pathway in response to Octopus TKRPs oct-TKRP I-VII with moderate ligand selectivity. Substance P and Octopus salivary gland-specific TK oct-TK-I completely failed to activate oct-TKRPR whereas a Substance P analog containing a C-terminal Arg-NH2 exhibited equipotent activation of oct-TKRPs. These functional analyses prove that oct-TKRPs but not oct-TK-I serve as endogenous functional ligands through oct-TKRPR although both of the family peptides were identified in a single species and the importance of C-terminal Arg-NH2 in the specific recognition of TKRPs by TKRPR is conserved through evolutionary lineages of Octopus. Southern blotting .

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