TAILIEUCHUNG - Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling

Analyses of integration of two-hormone signaling through the vertebrate nuclear hormone receptors, for which the retinoid X receptor is one part-ner, have generated a number of mechanistic models, including those des-cribed as ‘subordination’ models wherein ligand-activation of one partner is subordinate to the liganded state of the other partner. | ềFEBS Journal Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling Fang Fang2 Yong Xu1 Davy Jones2 and Grace Jones1 1 Department of Biology University of Kentucky Lexington KY USA 2 The Graduate Center for Toxicology University of Kentucky Lexington KY USA Keywords ecdysone receptor juvenile hormone methylepoxyfarnesoate retinoid-x-receptor ultraspiracle Correspondence G. Jones Department of Biology University of Kentuckey 304 Morgan Building Lexington KY 40506 USA Fax 1 859 257 7505 Tel 1 859 257 2105 E-mail gjones@ Received 14 September 2004 revised 13 January 2005 accepted 21 January 2005 doi Analyses of integration of two-hormone signaling through the vertebrate nuclear hormone receptors for which the retinoid X receptor is one partner have generated a number of mechanistic models including those described as subordination models wherein ligand-activation of one partner is subordinate to the liganded state of the other partner. However mechanisms by which two-hormone signaling is integrated through invertebrate nuclear hormone-binding receptors has not been heretofore experimentally elucidated. This report investigates the integration of signaling of invertebrate juvenile hormone JH and 20-OH ecdysone 20OHE at the level of identified nuclear receptors ultraspiracle and ecdysone receptor which transcriptionally activate a defined model core promoter JH esterase gene through specified hormone response elements DR1 and IR1 . Application of JH III or 20OHE to cultured Sf9 cells transfected with a DR1JHE-CoreLuciferase or reporter promoter each induced expression of the reporter. Cotreatment of transfected cells with both hormones yielded a greater than additive effect on transcription for especially the IR1JHECoreLuciferase reporter. Overexpression in Sf9 cells of recombinant Drosophila melanogaster ultraspiracle dUSP fostered formation

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