TAILIEUCHUNG - Báo cáo khoa học: Jackbean, soybean and Bacillus pasteurii ureases Biological effects unrelated to ureolytic activity

In this work we compared two plant ureases, jackbean urease (JBU) and embryo-specific soybean urease (SBU) and a bacterial (Bacillus pasteurii) urease, for kinetic parameters and other biological properties described recently for ureases that are independent of the ureolytic activity. The insecticidal effect of ureases was investigated in feedingtrials with the cotton sucker bug,Dysdercus peruvi-anus (Hemiptera) as an insect model. Contrastingwith B. pasteuriiurease (PBU), both plant ureases presented potent insecticidal activity, with LD50 values of (w/w) and (w/w) for JBU and SBU, respectively | Eur. J. Biochem. 271 1357-1363 2004 FEBS 2004 doi Jackbean soybean and Bacillus pasteurii ureases Biological effects unrelated to ureolytic activity Cristian Follmer Rafael Real-Guerra German E. Wasserman Deiber Olivera-Severo and Celia R. Carlini Department of Biophysics IB and Graduate Program in Cellular and Molecular Biology Center of Biotechnology Universidade Federal do Rio Grande do Sul Porto Alegre Brazil In this work we compared two plant ureases jackbean urease JBU and embryo-specific soybean urease SBU and a bacterial Bacillus pasteurii urease for kinetic parameters and other biological properties described recently for ureases that are independent of the ureolytic activity. The insecticidal effect of ureases was investigated in feeding mads with the cotton sucker bug Dysdercus peruvi-anus Hemiptera as an insect model. Contrasting with B. pasteurii urease PBU both plant ureases presented potent insecticidal activity with LD50 values of w w and w w for JBU and SBU respectively. The insecticidal property of JBU or SBU was not affected by treatment with p-hydroxymercuribenzoate an irreversible inhibitor of ureolytic activity of both proteins. Also contrasting with canatoxin - a ure cs e isol orm from jackbean seeds that displays a toxic effect in mice LD50 2 mg-kg-1 - no lethality was seen in mice injected intraperitoneally with JBU or SBU 20 mg-kg-1 . Similarly to canatoxin the three enzymes promoted aggregation of blood platelets EC50 itg-mL-1. itg-mL-1. itg-mL-1 for BPU SBU and JBU respectively . This platelet activating property was also independent of urease activity. Comparison of the kinetic properties indicated that SBU is fivefold less susceptible than JBU to inhibition by acetohydroxamic acid a chelator of Ni 2 and Zn 2 ions. The ureases also showed different susceptibility to agents that modify cysteine residues such as p-hydroxymercuribenzoate and p-benzo-quinone. Altogether these data

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• genetics
• Structural biochemistry
• Structural Biology
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine