TAILIEUCHUNG - Báo cáo khoa học: Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida)

NADH dehydrogenase activity was characterized in the mitochondrial lysates of Phytomonas serpens, a trypanosomatid flagellate parasitizing plants. Two different high molecular weight NADH dehydrogenases were characterized by native PAGE and detected by direct in-gel activity stain-ing. | ễFEBS Journal Characterization of the NADH ubiquinone oxidoreductase complex I in the trypanosomatid Phytomonas serpens Kinetoplastida Petra Cermáková1 Zdenek Verner2 Petr Man3 Julius Lukes2 and Anton Horvath1 1 Department of Biochemistry Faculty of NaturalSciences Comenius University Bratislava Slovakia 2 Biology Centre Institute of Parasitology Czech Academy of Sciences and Faculty of Biology University of South Bohemia Ceske Budejovice Budweis Czech Republic 3 Institute of Microbiology Czech Academy of Sciences Prague Czech Republic Keywords complex I NADH dehydrogenase Phytomonas respiratory chain trypanosomatid Correspondence A. Horvath Department of Biochemistry Faculty of Natural Sciences Comenius University Mlynska dolina CH-1 842 15 Bratislava Slovakia Fax 421 260296452 Tel 421 260296546 E-mail horvath@ Received 3 March 2007 revised 17 April 2007 accepted 25 April2007 NADH dehydrogenase activity was characterized in the mitochondrial lysates of Phytomonas serpens a trypanosomatid flagellate parasitizing plants. Two different high molecular weight NADH dehydrogenases were characterized by native PAGE and detected by direct in-gel activity staining. The association of NADH dehydrogenase activities with two distinct multisubunit complexes was revealed in the second dimension performed under denaturing conditions. One subunit present in both complexes crossreacted with the antibody against the 39 kDa subunit of bovine complex I. Out of several subunits analyzed by MS one contained a domain characteristic for the LYR family subunit of the NADH ubiquinone oxidoreduc-tases. Spectrophotometric measurement of the NADH ubiquinone 10 and NADH ferricyanide dehydrogenase activities revealed their different sensitivities to rotenone piericidin and diphenyl iodonium. doi NADH ubiquinone oxidoreductase complex I of the respiratory chain catalyzes the transfer of an electron pair from NADH to quinone an oxidation reaction that .

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