TAILIEUCHUNG - Báo cáo khoa học: RCAN1-1L is overexpressed in neurons of Alzheimer’s disease patients

At least two different isoforms ofRCAN1mRNA are expressed in neuro-nal cells in normal human brain. AlthoughRCAN1mRNA is elevated in brain regions affected by Alzheimer’s disease, it is not known whether the disease affects neuronal RCAN1, or if other cell types (. astrocytes or microglia) are affected. | ỊFEBS Journal RCAN1-1L is overexpressed in neurons of Alzheimer s disease patients Cathryn D. Harris Gennady Ermak and Kelvin J. A. Davies EthelPercy Andrus Gerontology Center and Division of Molecular ComputationalBiology The University of Southern California Los Angeles CA USA Keywords Alzheimer s disease calcipressin 1 DSCR1 Adapt78 RCAN1 Correspondence K. J. A. Davies EthelPercy Andrus Gerontology Center University of Southern California 3715 McClintock Avenue Los Angeles CA 90089-0191 USA Fax 1 213 740 6462 Tel 1 213 740 8959 E-mail kelvin@ Note The new name RCAN1 regulator of cal-cineurin has recently been accepted by the HUGO Gene Nomenclature Committee for the gene previously known as DSCR1 or Adapt78. Similarly RCAN1 is the new name for its protein product which was previously know as calcipressin 1 or MCIP1 Received 24 April2006 revised 16 December 2006 accepted 29 January 2007 doi At least two different isoforms of RCAN1 mRNA are expressed in neuronal cells in normal human brain. Although RCAN1 mRNA is elevated in brain regions affected by Alzheimer s disease it is not known whether the disease affects neuronal RCAN1 or if other cell types . astrocytes or microglia are affected. It is also unknown how many protein isoforms are expressed in human brain and whether RCAN1 protein is overexpressed in Alzheimer s disease. We explored the expression of both RCAN1-1 and RCAN1-4 mRNA isoforms in various cell types in normal and Alzheimer s disease postmortem samples using the combined technique of immunohistochemistry and in situ hybridization. We found that both exon 1 and exon 4 are predominantly expressed in neuronal cells and no significant expression of either of the exons was observed in astocytes or microglial cells. This was true in both normal and Alzheimer s disease brain sections. We also demonstrate that RCAN1-1 mRNA levels are approximately twofold higher in neurons from Alzheimer s disease patients versus

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