TAILIEUCHUNG - Báo cáo khoa học: The domains carrying the opposing activities in adenylyltransferase are separated by a central regulatory domain

Adenylyltransferase is a bifunctional enzyme that controls the enzymatic activity of dodecameric glutamine synthetase inEscherichia coliby rever-sible adenylylation and deadenylylation. Previous studies showed that the two similar but chemically distinct reactions are carried out by separate domains within adenylyltransferase. | ỊFEBS Journal The domains carrying the opposing activities in adenylyltransferase are separated by a central regulatory domain Paula Clancy1 Yibin Xu1 2 Wally C. van Heeswijk1 3 Subhash G. Vasudevan1 4 and David L. Ollis5 1 Department of Biochemistry and Molecular Biology James Cook University Townsville Australia 2 StructuralBiology Division The Walter and Eliza Hall Institute of MedicalResearch Parkville Australia 3 Department of Molecular CellPhysiology Faculty of Earth and Life Sciences Vrije Universiteit Amsterdam the Netherlands 4 Dengue Unit Novartis Institute for TropicalDiseases Singapore 5 Research Schoolof Chemistry Australian NationalUniversity Canberra Australia Keywords adenyltransferase intramolecular signaling monoclonalantibody regulatory domain Correspondence Y. Xu StructuralBiology Division The Walter and Eliza Hall Institute of Medical Research Parkville Victoria 3050 Australia Fax 61 3 9347 0852 Tel 61 3 9345 2305 E-mail xu@ Received 6 February 2007 revised 1 April 2007 accepted 4 April 2007 doi Adenylyltransferase is a bifunctional enzyme that controls the enzymatic activity of dodecameric glutamine synthetase in Escherichia coli by reversible adenylylation and deadenylylation. Previous studies showed that the two similar but chemically distinct reactions are carried out by separate domains within adenylyltransferase. The N-terminal domain carries the deadenylylation activity and the C-terminal domain carries the adenylyla-tion activity Jaggi R van Heeswijk WC Westerhoff HV Ollis DL Vasudevan SG 1997 EMBO J 16 5562-5571 . In this study we further map the domain junctions of adenylyltransferase on the basis of solubility and enzymatic analysis of truncation constructs and show for the first time that adenylyltransferase has three domains the two activity domains and a central probably regulatory R domain connected by interdomain Q-linkers N-Q1-R-Q2-C . The various constructs which have the opposing .

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