TAILIEUCHUNG - Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene

Streptomyces coelicolorandStreptomyces pilosusproduce desferrioxamine siderophores which are encoded by thedesABCDgene . pilosusis used for the production of desferrioxamine B which is utilized in human medicine. We report the deletion of thedesA gene encoding a lysine decarboxylase in Streptomyces coelicolorA3(2). | ễFEBS Journal Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene Sedef Tunca1 Carlos Barreiro1 Alberto Sola-Landa1 Juan Jose R. Coque1 2 and Juan F. Martin1 2 1 Institute de Biotecnologia INBIOTEC Leon Spain 2 Area de Microbiologia Facultad de CC Biologicas y Ambientales Universidad de Leon Spain Keywords desferrioxamine biosynthesis gene expression iron regulation lysine decarboxylase gene siderophores Correspondence J. F. Martin Instituto de Biotecnologia INBIOTEC Parque Cientifico de Leon Avenue delRealno. 1 24006 Leon Spain Fax 34 987 210 388 Tel 34 987 210 308 E-mail Received 11 October 2006 revised 19 December 2006 accepted 21 December 2006 doi Streptomyces coelicolor and Streptomyces pilosus produce desferrioxamine siderophores which are encoded by the desABCD gene cluster. S. pilosus is used for the production of desferrioxamine B which is utilized in human medicine. We report the deletion of the desA gene encoding a lysine decarboxylase in Streptomyces coelicolor A3 2 . The DdesA mutant was able to grow on lysine as the only carbon and nitrogen source but its des-ferrioxamine production was blocked confirming that the L-lysine decarboxylase encoded by desA is a dedicated enzyme committing L-lysine to desferrioxamine biosynthesis. Production of desferrioxamine was restored by complementation with the whole wild-type desABCD cluster but not by desA alone because of a polar effect of the desA gene replacement on expression of the downstream des genes. The transcription pattern of the desABCD cluster in S. coelicolor showed that all four genes were coordinately induced under conditions of iron deprivation. The transcription start point of the desA gene was identified by primer extension analysis at a thymine located 62 nucleotides upstream of the translation start codon. The -10 region of .

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