TAILIEUCHUNG - Báo cáo khoa học: Injection of poly(b-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate

Poly(b-L-malate) (PMLA) has been reported as an uncon-ventional, physiologically important biopolymer in plasmo-dia of myxomycetes, and has been proposed to function in the storage and transport of nuclear proteins by mimicking the phospho(deoxy)ribose backbone of nucleic acids. It is distributed in the cytoplasm and especially in the nuclei of these giant, multinucleate cells. We report here for the first time an increase in growth rate and a shortening of the cell cycle after the injection of purified PMLA. . | Eur. J. Biochem. 271 3805-3811 2004 FEBS 2004 doi Injection of poly b-L-malate into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate Michael Karl1 Roger Anderson2 and Eggehard Holler1 1Institut fur Biophysik und Physikalische Biochemie der Universitat Regensburg Germany 2Molecular Biology and Biotechnology University of Sheffield UK Poly PMLA has been reported as an unconventional physiologically important biopolymer in plasmodia of myxomycetes and has been proposed to function in the storage and transport of nuclear proteins by mimicking the phospho deoxy ribose backbone of nucleic acids. It is distributed in the cytoplasm and especially in the nuclei of these giant multinucleate cells. We report here for the first time an increase in growth rate and a shortening of the cell cycle after the injection of purified PMLA. By comparing two strains of Physarum polycephalum that differed in their production levels of PMLA it was found that growth activation and cell cycle shortening correlated with the relative increases of PMLA levels in the cytoplasm or the nuclei. Growth rates of a low PMLA producer strain LU897 X LU898 were increased by 40-50 while those of a high producer strain M3CVIII were increased by only 0-17 in comparison with controls. In both strains shortening of the cell cycle occurred to a similar extent and this was associated with similar increases in nuclear PMLA levels. The effects showed saturation dependences with regard to the amount of injected PMLA. A steep rise of intracellular PMLA shortly after injection was followed by the appearance of histone H1 in the cytoplasm. The increase in growth rate the shortening of the cell cycle duration and the appearance of H1 in the cytoplasm suggest that PMLA competes with nucleic acids in binding to proteins that control translation and or transcription. Thus PMLA could play an important role in the coordination

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