TAILIEUCHUNG - báo cáo khoa học: "Genetic variation of g-tocopherol methyltransferase gene contributes to elevated a-tocopherol content in soybean seeds"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Genetic variation of g-tocopherol methyltransferase gene contributes to elevated a-tocopherol content in soybean seeds | Dwiyanti et al. BMC Plant Biology 2011 11 152 http 1471 -2229 11 152 BMC Plant Biology RESEARCH ARTICLE Open Access Genetic variation of y-tocopherol methyltransferase gene contributes to elevated a-tocopherol content in soybean seeds Maria S Dwiyanti Tetsuya Yamada Masako Sato Jun Abe and Keisuke Kitamura Abstract Background Improvement of a-tocopherol content is an important breeding aim to increase the nutritional value of crops. Several efforts have been conducted to improve the a-tocopherol content in soybean Glycine max L. Merr. through transgenic technology by overexpressing genes related to a-tocopherol biosynthesis or through changes to crop management practices. Varieties with high a-tocopherol content have been identified in soybean germplasms. The heritability of this trait has been characterized in a cross between high a-tocopherol variety Keszthelyi Aproszemu Sarga KAS and low a-tocopherol variety Ichihime. In this study the genetic mechanism of the high a-tocopherol content trait of KAS was elucidated. Results Through QTL analysis and fine mapping in populations from a cross between KAS and a Japanese variety Ichihime we identified Ỵ-TMT3 which encodes tocopherol methyltransferase as a candidate gene responsible for high a-tocopherol concentration in KAS. Several nucleotide polymorphisms including two nonsynonymous mutations were found in the coding region of Ỵ-TMT3 between Ichihime and KAS but none of which was responsible for the difference in a-tocopherol concentration. Therefore we focused on transcriptional regulation of Ỵ-TMT3 in developing seeds and leaves. An F5 line that was heterozygous for the region containing y-TMT3 was self-pollinated. From among the progeny plants that were homozygous at the y-TMT3 locus were chosen for further evaluation. The expression level of y-TMT3 was higher both in developing seeds and leaves of plants homozygous for the Ỵ-TMT3 allele from KAS. The higher expression level was closely .

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