TAILIEUCHUNG - Báo cáo khoa học: Noninvasive imaging of microRNA124a-mediated repression of the chromosome 14 ORF 24 gene during neurogenesis

The function of microRNAs (miRNAs) is translational repression or mRNA cleavage of target genes by binding to 3¢-UTRs of target mRNA. In this study, we investigated the functions and the target genes of micro-RNA124a (miR124a), and imaged the miR124a-mediated repression of chromosome 14 open reading frame24(c14orf24, unknown function) during neurogenesis, using noninvasive luciferase systems. | Noninvasive imaging of microRNA124a-mediated repression of the chromosome 14 ORF 24 gene during neurogenesis Hae Young Ko1 2 3 Dong Soo Lee1 4 and Soonhag Kim5 1 Department of Nuclear Medicine SeoulNationalUniversity College of Medicine Korea 2 Interdisciplinary Course of Radiation Applied Life Science SeoulNationalUniversity College of Medicine Korea 3 Institute of Radiation Medicine MedicalResearch Center Seoul Korea 4 Department of Molecular Medicine and BiopharmaceuticalScience SeoulNationalUniversity College of Medicine Korea 5 Laboratory of Molecular Imaging CHA Stem Cell Institute CHA University Seoul Korea Keywords c14orf24 imaging microRNA124a neurogenesis target gene Correspondence S. Kim Laboratory of Molecular Imaging CHA Stem Cell Institute CHA University 605-21 Yoeksam 1-dong Gangnam-gu Seoul 135-081 Korea Fax 82 2 3468 3373 Tel 82 2 3468 2830 E-mail kimsoonhag@ D. S. Lee Department of Nuclear Medicine SeoulNationalUniversity College of Medicine 28 Yongon-dong Chongno-gu Seoul 110-744 Korea Fax 82 2 3668 7090 Tel 82 2 2072 2501 E-mail dsl@ Received 16 April2009 revised 15 June 2009 accepted 29 June 2009 doi The function of microRNAs miRNAs is translational repression or mRNA cleavage of target genes by binding to 3 -UTRs of target mRNA. In this study we investigated the functions and the target genes of micro-RNA124a miR124a and imaged the miR124a-mediated repression of chromosome 14 open reading frame24 c14orf24 unknown function during neurogenesis using noninvasive luciferase systems. The expression and functions of miR124a were investigated in neuronal differentiation of P19 cells P19 is a mouse embryonic carcinoma cell line by qRT-PCR and RT-PCR. The predicted target genes of miR124a were found by searching a bioinformatics database and confirmed by RT-PCR analysis. Remarkable repression of c14orf24 by miR124a was detected during neurogenesis and was imaged using in vitro and in vivo .

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