TAILIEUCHUNG - Báo cáo khoa học: Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death

Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death Paola Matarrese1, Elisabetta Straface1, Giuseppe Palumbo2, Maurizio Anselmi2, Lucrezia Gambardella1, Barbara Ascione1, Domenico Del Principe2 and Walter Malorni1 1 Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanita’, Rome, Italy 2 Department of Pediatrics, University of Rome Tor Vergata, Italy Keywords aggregation; apoptosis; mitochondrial membrane potential; platelets; zymosan Correspondence P. Matarrese, Department of Therapeutic Research and Medicines Evaluation, Section of Cell Aging and Degeneration, Istituto Superiore di Sanita’, viale Regina Elena 299, 00161 Rome, Italy Fax: +39 6 49903691 Tel: +39 6 49902010 E-mail:. | Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A - activation and long-term commitment to cell death Paola Matarrese1 Elisabetta Straface1 Giuseppe Palumbo2 Maurizio Anselmi2 Lucrezia Gambardella1 Barbara Ascione1 Domenico Del Principe2 and Walter Malorni1 1 Department of Therapeutic Research and Medicines Evaluation Istituto Superiore di Sanita Rome Italy 2 Department of Pediatrics University of Rome Tor Vergata Italy Keywords aggregation apoptosis mitochondrial membrane potential platelets zymosan Correspondence P. Matarrese Department of Therapeutic Research and Medicines Evaluation Section of CellAging and Degeneration Istituto Superiore di Sanita viale Regina Elena 299 00161 Rome Italy Fax 39 6 49903691 Tel 39 6 49902010 E-mail Received 1 November 2008 revised 25 November 2008 accepted 3 December 2008 doi Changes in the mitochondrial membrane potential play a key role in determining cell fate. Mitochondria membrane hyperpolarization has been found to occur after cell activation . in lymphocytes whereas depolarization is associated with apoptosis. The aim of this study was to investigate the effects of an immunological stimulus . opsonized zymosan A on human platelet mitochondria by means of flow and static cytometry analyses as well as biochemical methods. We found that opsonized zymosan induced significant changes of platelet morphology at early time points 90 min . This was associated with increased production of reactive oxygen species and intriguingly mitochondrial membrane hyperpolarization. At a later time point 24 h opsonized zymosan was found to induce increased expression of CD47 adhesion molecule platelet aggregation mitochondrial membrane depolarization and phosphatidylserine externalization. Although these late events usually represent signs of apoptosis in nucleated cells in opsonized zymosan-treated platelets they were not associated with membrane integrity .

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