TAILIEUCHUNG - Báo cáo lâm nghiệp: " A flow cytometric evaluation of the nuclear DNA content and GC percent in genomes of European oak species"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế, đề tài: " A flow cytometric evaluation of the nuclear DNA content and GC percent in genomes of European oak species. | Ann Sci For 1996 53 915-917 Elsevier INRA 915 Short note A flow cytometric evaluation of the nuclear DNA content and GC percent in genomes of European oak species JM Favre1 s Brown2 1 Laboratoire de biologie forestiere associé Inra faculte des sciences BP 239 54506 Vandceuvre-lès-Nancy cedex zCytométrie Institut des sciences végétales CNRS UPR 40 91198 Gif-sur-Yvette France Received 18 May 1995 accepted 7 December 1995 Summary - The nuclear DNA content and GC have been assessed for the first time by flow cytometry in Quercus petraea Matt Liebl o pubescens wind and QroburL. Values were respectively 2C and pg with and GC . These estimates of DNA content were greater than those previously obtained by Feulgen microdensitometry. This difference is discussed in reference to effects of tanning in sample preparation. nuclear DNA content GC percent European oaks flow cytometry Resume - Evaluation par cytométrie en flux du contenu en ADN nucléaire et du pourcentage de GC Chez des chênes européens. Le contenu en ADN nucléaire et le pourcentage de GC ont été évalués pour la premiere fols par cytométrie en flux Chez Quercus petraea Matt Liebl Q pubescens Willd and Q robur L. Les valeurs enregistrées respectivement 1 87 1 86 et 1 84 pg avec 41 7 42 1 et 42 0 GC sont comparables pour les trois espèces mais les contenus en ADN se sont révélés supérieurs à ceux obtenus antérieurement par microdensitométrie. Cette difference est discutée en relation avec les effets de tannage tors de la preparation des échantillons. contenu en ADN nucléaire pourcentage de GC chênes européens cytométrie en flux INTRODUCTION Karyotypic studies in genus Quercus Fa-gaceae have shown that the species are diploid 2n 2x 24 Darlington and Wylie 1955 Moore 1982 although occasional polyploids have been found Butorina 1993 . Recently a detailed karyo-morphological analysis in Q petraea Matt Liebl Q robur L and Q rubra L showed that the different chromosomes can be identified and .

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