TAILIEUCHUNG - Akt regulates the expression of MafK, synaptotagmin I, and syntenin-1, which play roles in neuronal function

Akt regulates various cellular processes, including cell growth, survival, and metabolism. Recently, Akt's role in neurite outgrowth has also emerged. We thus aimed to identify neuronal function-related genes that are regulated by Akt. Methods: We performed suppression subtractive hybridization on two previously established PC12 sublines, one of which overexpresses the wild-type (WT) form and the other, the dominant-negative (DN) form of Akt. These sublines respond differently to NGF's neuronal differentiation effect. Results: A. | Ro et al. Journal of Biomedical Science 2010 17 18 http content 17 1 18 I NSC The cost of publication in Journal of Biomedical Science is bourne by the National Science Council Taiwan. JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Akt regulates the expression of MafK synaptotagmin I and syntenin-1 which play roles in neuronal function Young-Tae Ro1 Bo-Kwang Jang2 Chan Young Shin2 Eui U Park3 Chul Geun Kim5 and Sung-Il Yang 2 4 Abstract Background Akt regulates various cellular processes including cell growth survival and metabolism. Recently Akt s role in neurite outgrowth has also emerged. We thus aimed to identify neuronal function-related genes that are regulated by Akt. Methods We performed suppression subtractive hybridization on two previously established PC12 sublines one of which overexpresses the wild-type WT form and the other the dominant-negative DN form of Akt. These sublines respond differently to NGF s neuronal differentiation effect. Results A variety of genes was identified and could be classified into several functional groups one of which was developmental processes. Two genes involved in neuronal differentiation and function were found in this group. v-Maf musculoaponeurotic fibrosarcoma oncogene homolog K MafK induces the neuronal differentiation of PC12 cells and immature telencephalon neurons and synaptotagmin I SytI is essential for neurotransmitter release. Another gene syntenin-1 Syn-1 was also recognized in the same functional group into which MafKand SytI were classified. Syn-1 has been reported to promote the formation of membrane varicosities in neurons. Quantitative reverse transcription polymerase chain reaction analyses show that the transcript levels of these three genes were lower in PC12 WT-Akt cells than in parental PC12 and PC12 DN-Akt cells. Furthermore treatment of PC12 WT-Akt cells with an Akt inhibitor resulted in the increase of the expression of these genes and the improvement of neurite outgrowth.

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