TAILIEUCHUNG - Handbook of Microbiological Media, Fourth Edition part 32

Handbook of Microbiological Media, Fourth Edition part 32 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Campylobacter Medium 305 Plant extract No. Horse blood Selectrive supplement pH at 25 C Source This medium without horse blood and antibiotic supplement is available as a premixed powder from HiMedia. Selective Supplement Solution Composition per Amphotericin Polymyxin B U Preparation of Selective Supplement Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except horse blood and selective supplement to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add of lysed horse blood and of sterile selective supplement. Mix thoroughly. Pour into sterile Petri dishes. Use For the cultivation of Campylobacter species. Normally used with a selective supplement to suppress the growth of other bacterial species. Campylobacter Isolation Agar A Composition per liter Beef Casein Yeast Sodium Sodium Antibiotic pH at 25 C Antibiotic Solution Composition per Sodium Preparation of Antibiotic Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium Add components except antibiotic solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile antibiotic solution. Mix thoroughly. Pour into sterile Petri .

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