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Handbook of Microbiological Media, Fourth Edition part 32 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Campylobacter Medium 305 Plant extract No. 2.2.5g Horse blood lysed.50.0mL Selectrive supplement solution.10.0mL pH 7.4 0.2 at 25 C Source This medium without horse blood and antibiotic supplement is available as a premixed powder from HiMedia. Selective Supplement Solution Composition per 10.0mL Cephalothin.15.0mg Vancomycin.10.0mg Trimethoprim.5.0mg Amphotericin B.2.0mg Polymyxin B sulfate.2500 U Preparation of Selective Supplement Solution Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except horse blood and selective supplement to distilled deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 50.0mL of lysed horse blood and 10.0mL of sterile selective supplement. Mix thoroughly. Pour into sterile Petri dishes. Use For the cultivation of Campylobacter species. Normally used with a selective supplement to suppress the growth of other bacterial species. Campylobacter Isolation Agar A Composition per liter Agar.12.0g Beef extract.10.0g Peptone.10.0g NaCl.5.0g Charcoal.4.0g Casein hydrolysate.3.0g Yeast extract.2.0g Sodium deoxycholate.1.0g FeSO4.0.25g Sodium pyruvate.0.25g Antibiotic solution.10.0mL pH 7.4 0.2 at 25 C Antibiotic Solution Composition per 10.0mL Cycloheximide.0.1g Sodium cefoperazone.0.03g Preparation of Antibiotic Solution Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium Add components except antibiotic solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile antibiotic solution. Mix thoroughly. Pour into sterile Petri .
