TAILIEUCHUNG - Báo cáo sinh học: "Mapping the porcine RN gene to chromosome 15"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Mapping the porcine RN gene to chromosome 15 | Genet Sei Evol 1996 28 437-442 Elsevier INRA 437 Original article Mapping the porcine RN gene to chromosome 15 c Looft N Reinsch I Rudat E Kalm Institute of Animal Breeding and Husbandry Christian-Albrechts University of Kiel Olshausenstrasse 40 24118 Kiel Germany Received 6 October 1995 accepted 4 July 1996 Summary - Microsatellite markers have been used to map the porcine RN gene to chromosome 15 as previously reported. This locus affects the technological quality of meat in pigs. Resource families were established by mating 16 crossbred boars Hampshire X Piétrain to 65 crossbred sows Large White X Landrace . From 564 progeny meat samples were taken from the musculus longissimus dorsi 24 h post mortem and the glycogen concentration was determined. The assessment of RN genotypes is based on the measurement of the glycogen concentration. Boars were heterozygous RN rn and sows were homozygous rn rn at the RN locus. A subsample of 263 animals was typed with four microsatellite markers from chromosome 15. Linkage analysis confirmed that the RN locus maps to chromosome 15 and showed linkage to Sw936 at a distance of 4 cM. pig meat quality glycogen microsatellite marker linkage Resume La localisation du gène RN porcin sur le chromosome 15. Des marqueurs micro satellites ont été utilises pour localiser le gène RN du pore sur le chromosome 15 dans une étude antérieure. Ce locus influence la qualité technologique de la viande. Les families étudiêes ici ont ếté construites par accouplement de 16 verrats croisés Hampshire X Piétrain avec 65 truies croisées Large White X Landrace . Des échantillons de viande de 564 descendants ont été extraits du muscle longissimus dorsi 24 heures post mortem pour mesurer la concentration en glycogène. La determination du genotype RN est basée sur la valeur de la concentration en glycogène. Les verrats étaient heterozygotes RN rn et les truies homozygotes rn l rn au locus RN. Une sous-population de 263 animaux a été typée à I aide de quatre .

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