TAILIEUCHUNG - Báo cáo sinh học: "CGB and GNRH1 expression analysis as a method of tumor cells metastatic spread detection in patients with gynecological malignances"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: CGB and GNRH1 expression analysis as a method of tumor cells metastatic spread detection in patients with gynecological malignances | Andrusiewicz et al. Journal of Translational Medicine 2011 9 130 http content 9 1 130 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access CGB and GNRH1 expression analysis as a method of tumor cells metastatic spread detection in patients with gynecological malignances Miroslaw Andrusiewicz1 Anna Szczerba1 Maria Wolun-Cholewa1 Wojciech Warchol2 Ewa Nowak-Markwitz3 Emilia G siorowska3 Krystyna Adamska4 and Anna Jankowska1 Abstract Background Metastasis is a common feature of many advanced stage cancers and metastatic spread is thought to be responsible for cancer progression. Most cancer cells are localized in the primary tumor and only a small population of circulating tumor cells CTC has metastatic potential. CTC amount reflects the aggressiveness of tumors therefore their detection can be used to determine the prognosis and treatment of cancer patients. The aim of this study was to evaluate human chorionic gonadotropin beta subunit CGB and gonadoliberin type 1 GNRH1 expression as markers of tumor cells circulating in peripheral blood of gynecological cancer patients indicating the metastatic spread of tumor. Methods CGB and GNRH1 expression level in tumor tissue and blood of cancer patients was assessed by real-time RT-PCR. The data was analyzed using the Mann-Whitney U and Spearman tests. In order to distinguish populations with homogeneous genes expression the maximal likelihood method for one- and multiplied normal distribution was used. Result Real time RT-PCR results revealed CGB and GNRH1 genes activity in both tumor tissue and blood of gynecological cancers patients. While the expression of both genes characterized all examined tumor tissues in case of blood analysis the transcripts of GNRH1 were found in all cancer patients while CGB were present in 93 of patients. CGB and GNRH1 activity was detected also in control group which consisted of tissue lacking cancerous changes and blood of healthy volunteers. The .

TÀI LIỆU LIÊN QUAN
TAILIEUCHUNG - Chia sẻ tài liệu không giới hạn
Địa chỉ : 444 Hoang Hoa Tham, Hanoi, Viet Nam
Website : tailieuchung.com
Email : tailieuchung20@gmail.com
Tailieuchung.com là thư viện tài liệu trực tuyến, nơi chia sẽ trao đổi hàng triệu tài liệu như luận văn đồ án, sách, giáo trình, đề thi.
Chúng tôi không chịu trách nhiệm liên quan đến các vấn đề bản quyền nội dung tài liệu được thành viên tự nguyện đăng tải lên, nếu phát hiện thấy tài liệu xấu hoặc tài liệu có bản quyền xin hãy email cho chúng tôi.
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.