TAILIEUCHUNG - Báo cáo sinh học: "Optimised electroporation mediated DNA vaccination for treatment of prostate cancer"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Optimised electroporation mediated DNA vaccination for treatment of prostate cancer | Ahmad et al. Genetic Vaccines and Therapy 2010 8 1 http content 8 1 1 GENETIC VACCINES AND THERAPY RESEARCH Open Access Optimised electroporation mediated DNA vaccination for treatment of prostate cancer c rCr 7 Ah m h 1 2 3 C r r Wl- f Jr2 D -s I I I Ct A r1 2 3 h z -s r I I s u- Z A U . r 1 2 r V I h C h c I 11 I I f-s 1 2 DdiTidZ Ahmdd Gdrrett Cdsey Pdul Sweeney MdiK Tdngney Gerdld C OSullivdn Abstract Background Immunologicdl therapies enhdnce the dbility oT the immune system to recognise dnd destroy cdncer cells vid selective Killing mechdnisms. DNA vdccines hdve potentidl to dctivdte the immune system dgdinst specific dntigens with dccompdnying potent immunologicdl ddjuvdnt effects from unmethyldted CpG motifs ds on proKdryotic DNA. We investigdted dn electroporation driven pldsmid DNA vdccindtion strategy in dnimdl models for tredtment of prostdte cdncer. Methods Pldsmid expressing humdn PSA gene phPSA wds delivered in vivo by intrd-musculdr electroporation to induce effective dnti-tumour immune responses dgdinst prostdte dntigen expressing tumours. Groups of mdle C57 BL 6 mice received intrd-musculdr injections of phPSA pldsmid. For phPSA delivery quddriceps muscle wds injected with 50 pg pldsmid. After 80 seconds squdre-wdve pulses were ddministered in sequence using d custom designed pulse generator dnd dcustom-designed dpplicdtor with 2 needles pldced through the skin central to the muscle. To determine dn optimum tredtment regimen three different vdccindtion schedules were investigdted. In d sepdrdte experiment the immune potentidl of the phPSA vdccine wds further enhdnced with co- ddministrdtion of synthetic CpG rich oligonucleotides. One weeK dfter ldst vdccindtion the mice were chdllenged subcutdneously with TRAMPC1 hPSA prostdte cdncer cell line stdbly expressing humdn PSA dnd tumour growth wds monitored. Serum from dnimdls wds exdmined by ELISA for dnti-hPSA dntibodies dnd for IFNg. Histologicdl dssessment of the tumours wds .

• Báo cáo khoa học
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học