TAILIEUCHUNG - Báo cáo sinh học: "∆RR vaccination protects from KA-induced seizures and neuronal loss through ICP10PK-mediated modulation of the neuronal-microglial axis"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: ∆RR vaccination protects from KA-induced seizures and neuronal loss through ICP10PK-mediated modulation of the neuronal-microglial axis | Genetic Vaccines and Therapy BioMed Central Research Open Access ARR vaccination protects from KA-induced seizures and neuronal loss through ICP10PK-mediated modulation of the neuronal-microglial axis Jennifer M Laing and Laure Aurelian Address Department of Pharmacology and Experimental Therapeutics University of Maryland School of Medicine Baltimore MD 21201 USA Email Jennifer M Laing - jlain001@ Laure Aurelian - laurelia@ Corresponding author Published 7 January 2008 Received 10 September 2007 Genetic Vaccines and Therapy 2008 6 1 doi 186 1479-0556-6-1 Accepted 7 January 2008 This article is available from http content 6 1 1 2008 Laing and Aurelian licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Ischemic brain injury and epilepsy are common neurodegenerative diseases caused by excitotoxicity. Their pathogenesis includes microglial production of inflammatory cytokines. Our studies were designed to examine whether a growth compromised HSV-2 mutant ARR prevents excitotoxic injury through modulation of microglial responses by the anti-apoptotic HSV-2 protein ICP10PK. EOC2 and EOC20 microglial cells which are differentially activated were infected with ARR or the ICP10PK deleted virus APK and examined for virus-induced neuroprotective activity. Both cell lines were non-permissive for virus growth but expressed ICP10PK ARR or the PK deleted ICP10 protein p95 APK . Conditioned medium CM from ARR- but not APK-infected cells prevented N-methyl-D-aspartate NMDA -induced apoptosis of primary hippocampal cultures as determined by TUNEL and caspase-3 activation neuroprotection . Neuroprotection was associated with inhibition of TNF-a and RANTES and production of .

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