TAILIEUCHUNG - Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis

Altered dopamine homeostasis is an accepted mechanism in the pathogene-sis of Parkinson’s disease. a-Synuclein overexpression and impaired dis-posal contribute to this mechanism. However, biochemical alterations associated with the interplay of cytosolic dopamine and increaseda-synuc-lein are still unclear. | ỊFEBS Journal Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson s disease pathogenesis Tiziana Alberio1 Alessandra Maria Bossi2 Alberto Milli2 Elisa Parma1 Marzia Bruna Gariboldi1 Giovanna Tosi3 Leonardo Lopiano4 and Mauro Fasano1 1 Department of Structuraland FunctionalBiology and Centre of Neuroscience University of Insubria Busto Arsizio Italy 2 Department of Biotechnology University of Verona Italy 3 Department of Clinicaland BiologicalSciences University of Insubria Varese Italy 4 Department of Neuroscience University of Torino Italy Keywords dopamine network enrichment NF-kB Parkinson s disease SH-SY5Y a-synuclein Correspondence M. Fasano Department of Structural and FunctionalBiology and Centre of Neuroscience University of Insubria via Alberto da Giussano 12 21052 Busto Arsizio Italy Fax 39 0331 339459 Tel 39 0331 339450 E-mail Website http cns fasano Received 4 June 2010 revised 14 July 2010 accepted 27 September 2010 doi Altered dopamine homeostasis is an accepted mechanism in the pathogenesis of Parkinson s disease. a-Synuclein overexpression and impaired disposal contribute to this mechanism. However biochemical alterations associated with the interplay of cytosolic dopamine and increased a-synuc-lein are still unclear. Catecholaminergic SH-SY5Y human neuroblastoma cells are a suitable model for investigating dopamine toxicity. In the present study we report the proteomic pattern of SH-SY5Y cells overexpressing a-synuclein induction after dopamine exposure. Dopamine itself is able to upregulate a-synuclein expression. However the effect is not observed in cells that already overexpress a-synuclein as a consequence of transfection. The proteomic analysis highlights significant changes in 23 proteins linked to specific cellular processes such as cytoskeleton structure and regulation mitochondrial function energetic .

• Báo cáo khoa học
• Scientific reports
• breast cancer
• medicine
• Parkinson
• synuclein
• Proteomic analysis
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes