TAILIEUCHUNG - Báo cáo y học: " Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood"

Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood | Esposito et al. Virology Journal 2010 7 85 http content 7 1 85 VIROLOGY JOURNAL SHORT REPORT Open Access Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood Susanna Esposito 1 Claudio G Molten 1 Cristina Daleno1 Antonia Valzano1 Claudia Tagliabue1 Carlotta Galeone2 Gregorio Milani1 Emilio Fossali1 Paola Marchisio1 and Nicola Principi 1 Abstract This study evaluated the efficiency of pediatric mid-turbinate nasal flocked swabs used by parents in 203 children aged 6 months to 5 years with signs and symptoms of respiratory disease. Two nasal samples were collected from each child in a randomised sequence one by a trained pediatrician and one by a parent. The real-time polymerase chain reaction influenza virus detection rates were similar in the samples collected using the two methods Cohen s kappa as were the cycle threshold values. In comparison with the pediatrician-collected samples the sensitivity and specificity of the parental collections were respectively 95 confidence interval CI and 95 CI and the positive and negative predictive values were respectively 95 CI and 95 CI . The children were significantly more satisfied with the parental collections median values standard deviation vs p . These findings show that mid-turbinate nasal flocked swabs specifically designed for infants and children can be used by parents without reducing the influenza virus detection rate. Moreover the direct involvement of parents significantly increases patient acceptance thus simplifying collection and suggesting that this novel swab design should be considered for epidemiological surveys and vaccine efficacy studies. Finding In order to monitor the circulation of infectious agents and evaluate the efficacy of specific vaccines it is essential to be able to identify the viruses that cause .

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