TAILIEUCHUNG - Drugs and Poisons in Humans - A Handbook of Practical Analysis (Part 50)

Introduction: Ethylene glycol is being widely used as solvents, lubricants and surfactants in industries; for daily necessities, it is being used as a component of cold-reserving materials and of nonfreezing coolants of automobiles. There were cases of accidental and suicidal ingestion of such fluids [1–3]. As toxic actions of ethylene glycol, suppression of central nervous system activities similar to that of alcohol and metabolic acidosis caused by glycolic acid produced from ethylene glycol can be mentioned. The glycolic acid is further metabolized into oxalic acid, which is bound with calcium ions to form the insoluble salt; the salt precipitates in. | Ethyleneglycol by Kanako Watanabe Introduction Ethylene glycol is being widely used as solvents lubricants and surfactants in industries for daily necessities it is being used as a component of cold-reserving materials and of nonfreezing coolants of automobiles. There were cases of accidental and suicidal ingestion of such fluids 1-3 . As toxic actions of ethylene glycol suppression of central nervous system activities similar to that of alcohol and metabolic acidosis caused by glycolic acid produced from ethylene glycol can be mentioned. The glycolic acid is further metabolized into oxalic acid which is bound with calcium ions to form the insoluble salt the salt precipitates in various tissues. Especially such precipitation sometimes causes renal dysfunction failure 4 . For analysis of ethylene glycol methods by GC 5-8 GC MS 9-11 and HPLC 12-14 were reported. In this chapter a method of GC MS analysis of ethylene glycol in human plasma is presented. Reagents and their preparation i. Reagents Ethylene glycol and phenylboronic acid can be purchased from Sigma St. Louis MO USA and other manufacturers ethylene glycol-d4 from Aldrich Milwaukee WI USA No. 34 744-2 a. Other common chemicals used were of the highest purity commercially available. ii. Preparation After sampling venous blood with the vacuum tubes containing an anticoagulant they are centrifuged at 3 000 rpm for 5 min to obtain blood plasmab. Ethylene glycol-d4 IS solution a 8-pL aliquot of ethylene glycol-d4 specific gravity is dissolved in 50 mL acetonitrile. Ethylene glycol standard solution a 9-pL of ethylene glycol specific gravity is dissolved in 1 mL acetonitrile 100 pg 10 pL . Derivatization solution a aliquot of phenylboronic acid is dissolved in 1 mL acetonitrile. GC MS conditions GC column c an Rtx-17 fused silica medium-bore capillary column 30 m x mm i. d. film thickness pm Restek Bellefonte PA USA . GC conditions a GC-17A gas chromatographd Shimadzu .

• Y học thường thức
• Drugs and Poisons in Humans
• Handbook of Practical Analysis
• tác hại của ma tuý
• chất độc hại cho con người
• phòng chống nghiện ma tuý
• Ethylene glycol
• computer system for diagnosis
• causative drugs
• poisons developed
• Alternative specimens
• Pretreatments of human specimens
• Detection methods
• Practical use
• the poison net developed
• Problems in toxin analysis
• emergency medicine
• Analysis of chemical warfare agents
• related compounds
• Carbon monoxide
• Hydrogen sulfide
• metabolite
• Cyanide
• Methanol and formic acid
• Ethanol
• Chloroform and dichloromethane
• Toluene
• benzene
• xylene
• styrene
• Alkyl nitrites
• Components of gasoline
• Amphetamines
• metabolites
• Cannabinoids
• Morphine and its analogues
• Cocaine and its metabolites
• Pentazocine
• Lysergic acid diethylamide
• Methylenedioxyamphetamines