TAILIEUCHUNG - Extracellular vesicles mediated proinflammatory macrophage phenotype induced by radiotherapy in cervical cancer

Radiotherapy is a highly effective treatment for cervical cancer. Recent studies focused on the radiotherapy induced anti-tumor immunity. Whether tumor-derived extracellular vesicles (EVs) play roles in radiotherapy induced tumor associated macrophage (TAM) polarization remains unclear. | Ren et al. BMC Cancer 2022 22 88 https s12885-022-09194-z RESEARCH Open Access Extracellular vesicles mediated proinflammatory macrophage phenotype induced by radiotherapy in cervical cancer Junli Ren1 Lili Li1 Baofeng Yu2 Enwei Xu3 Naiping Sun1 Xiaoning Li2 Zihan Xing2 Xiaodong Han1 Yaqin Cui1 Xiaoyan Wang4 Xiaoxue Zhang5 and Guoliang Wang6 Abstract Background Radiotherapy is a highly effective treatment for cervical cancer. Recent studies focused on the radio- therapy induced anti-tumor immunity. Whether tumor-derived extracellular vesicles EVs play roles in radiotherapy induced tumor associated macrophage TAM polarization remains unclear. Materials and Methods This study analysed the phenotype of macrophages in cancer tissue and peripheral blood of cervical cancer patients using flow cytometry analysis. The role of EVs from plasma of post-irradiated patients on M2-like transformed macrophages was assessed. The M1- and M2-like macrophages were assessed by expression of cell surface markers CCR7 CD163 and intracellular cytokines IL-10 TNFα and iNOS . The capacity of phagocytosis was assessed by PD-1 expression and phagocytosis of pHrodo Red E. coli bioparticles. Results Our results demonstrated that radiotherapy of cervical cancer induced an increase in the number of TAMs and a change in their subtype from the M2-like to the M1-like phenotype increased expression of CCR7 and decreased expression of CD163 . The EVs from plasma of post-irradiated patients facilitated the M2-like to the M1-like phenotype transition increased expression of CCR7 TNFα and iNOS and decreased expression of CD163 and IL-10 and increased capacity of phagocytosis decreased PD-1 expression and increased phagocytosis of pHrodo Red E. coli bioparticles . Conclusions Our data demonstrated that irradiation in cervical cancer patients facilitated a proinflammatory macrophage phenotype which could eventually able to mediate anti-tumor immune responses. Our findings high- light the

• Y khoa - Dược
• BMC Cancer
• Extracellular vesicle
• Cervical cancer
• Tumor associated macrophage
• Polarization remains unclear
• Radiotherapy induced anti tumor immunity
• Cancer patient pathways
• Colorectal cancer
• Lung cancer
• Breast cancer
• Prostate cancer
• Ovarian cancer
• Cancer detection
• Ovarian cancer symptoms
• Ovarian cancer signs
• Ovarian cancer tests
• Cancer biomarkers
• Pediatric cancer
• Familial cancer predisposition
• Hereditary cancer syndrome
• Genetic cancer susceptibility
• Advanced cancer
• Biliary tract cancer
• Multimodal intervention
• Pancreatic cancer
• Physical activity
• Breast cancer screening
• Familial breast cancer
• Young breast cancer survivors
• High risk relatives
• Randomized trial
• Childhood cancer
• Childhood cancer survivor
• Thyroid cancer screening
• Secondary cancer
• Cancer screening
• Community awareness
• Cancer education
• Prior cancer
• Cancer survivors
• Cancer registry
• Second cancer
• Surgical oncology
• Cancer imaging
• Radiation oncologists
• Medical oncologists
• Oesophagogastric cancer
• Liver cancer
• Hepatobiliary cancer
• Multidisciplinary rehabilitation
• EGFR mutation
• Family history of cancer
• Inherited susceptibility
• Cancer predisposition genes
• Lung cancer aetiology
• Cancer stem cells
• Oct4 overexpressing lung cancer
• Human lung cancer xenograft model
• Disease free survivals
• Cancer hallmarks
• Cancer genesis
• Defiant disease
• Mutational signature
• Cancer diagnosis
• Cancer genomics
• Molecular medicine
• Whole genome sequencing
• Cancer susceptibility gene
• Breast cancer prognosis
• Next generation sequencing
• Quality of care
• Quality indicators
• Shifting cancer care
• German cancer society
• Colorectal cancer screening
• Stomach cancer screening
• Lung cancer survivor
• Physician recommendation
• Total number of lymph nodes
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• Overall survival
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• Young onset
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• Risk factor
• Early detection of cancer
• Gastric cancer incidence
• Population based specialized registry
• Gastric cancer survival
• Gastric cancer clinical characteristics
• Gastric cancer pathological characteristics
• Lung Cancer Screening
• Racial disparities
• Lung Cancer diagnosis
• Screening adherence
• Second primary colorectal cancer
• Standardized incidence ratio
• Left sided colon cancer
• Right sided colon cancer
• Quality of cancer care
• Population based study