TAILIEUCHUNG - Purification of industrially important Proteases from Pseudomonas strain and its kinetic studies

The aim of study was development of industrially and agriculturally important bacterial strains which produces antimicrobial protease. It is a kind of study that tends to contribute the potential Pseudomonas strains that may be helpful as plant growth promoting, biocontrol agent and may be used for the production of antimicrobial protease at industrial level. In the present study we have tried to isolate the fluorescent Pseudomonas sp. from three medicinal plants ., Achyranthus aspera, Elaegnus umbellate and Heracleum candicans. Three strains were isolated ., A4, H5 and E6, which were showed the high production of antibacterial, antifungal and proteolytic activity. The enzyme protease was purified from the strain H5 by using Sephadex G-75 column chromatography. Kinetic study of the purified protease was done by checking the effect of pH and temperature, substrate concentration, enzyme concentration and reaction time. Purified proteases can be exploited for industrial use as it is in high demands in different industries. | Purification of industrially important Proteases from Pseudomonas strain and its kinetic studies

• Sinh học
• Pseudomonas antimicrobial
• Antifungal activity
• Industrially important Proteases
• Kinetic studies
• Pseudomonas strain and its kinetic studies
• Pseudomonas aeruginosa
• Extended spectrum beta lactamase
• Non extended spectrum beta lactamase
• Producing pseudomonas aeruginosa
• Occurrence of antimicrobial resistance
• Screening antimicrobial potential
• Copper nanoparticles
• Antimicrobial ability
• Pseudomonas fluorescens
• Bacillus subtilis
• Pseudomonas aeruginosa (P aeruginosa)
• Antimicrobial resistance pattern
• Various clinical samples
• Government medical college
• Otitis media
• Antimicrobial susceptibility testing
• Kirby bauer disk diffusion
• Antimicrobial resistance
• Staphylococcus aureus
• Antimicrobial effects
• Healthy people
• Electrospray ionization
• Antibiotic policies
• Infection control practices
• Tertiary care hospital
• Antimicrobial susceptibility patter
• Antimicrobial sensitivity testing
• Drug resistance
• Medical tips
• Blood from patients
• Pseudomonas aeruginosa recovered
• BMC Genomics
• Pseudomonas putida
• Horizontal gene transfer
• Gram negative
• Non fermenting bacterium
• Carbohydrate polymers
• Multidrug resistant bacteria
• Antimicrobial peptide dendrimers
• Antimicrobial formulations
• Wound infections
• Dexamethasone abrogates
• Antibiofilm activities
• Different drugs against clinical isolates
• Surgical site infection
• Tertiary care centre
• Prevalence of pseudomonas aeruginosa
• Standard microbiological techniques
• Antimicrobial activity
• Antioxidant activity
• Phenolic compounds
• Staphylococcus aureus ATCC 29213
• Mycobacterium smegmatis
• Extended spectrum beta lactamases
• Post operative wound infection
• Antimicrobial susceptibility
• Journal of Chemistry
• Pyridazino[4 5 b]indole
• β carboline
• Escherichia coli ATCC 25922
• Pseudomonas aeruginosa ATCC 27853
• 2 Pyrazoline thiazole
• Pseudomonas aeruginosa (ATCC 27853)
• Escherichia coli (ATCC 35218)
• Palustriella commutata
• Yersinia enterocolitica
• Escherichia coli
• Ceratonia siliqua L
• Cytotoxic activity
• Shrimp lethality bioassay
• Pseudomonas aeroginosa
• Viscum album subsp
• Plant extract
• Enterobacter cloacae
• Scientific reports
• scientific research
• biochemistry
• Crystal structure
• Antimicrobial
• biosynthesis
• thesis report
• medical report
• immune system
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học
• Report medicine
• traditional medicine
• cell structure
• genetics