TAILIEUCHUNG - Molecular characterization of potent biocontrol isolates of Trichoderma asperellum and Pseudomonas fluorescens from tomato rhizosphere

The potent native bioagents isolated from tomato rhizosphere have high antagonistic potential against Phytophthora infestans. Molecular characterization of fungal isolates (T-11 and T-14) was performed by using Internal Transcribed Spacer (ITS) region of 18S rRNA sequence. The sequence analyses of two isolates (MK928414 and MK928417), showed 100% similarity with Trichoderma asperellum during BLAST analysis. The phylogeny tree constructed by using sequence analysis showed the formation of cluster along with T. asperellum and both the isolates were identified as T. asperellum. The rhizospheric potential bacterial isolates (Pf-2 and Pf-3) were isolated and characterized at molecular level. Bacterial isolates were identified as Pseudomonas fluorescens by using 16S-23S rRNA intervening sequence specific ITS1 and ITS2 primers. The sequence analyses of two isolates showed similarity with P. fluorescens during BLAST analysis and received the NCBI GenBank accession number of MN783298 and MN783297 for Pf-2 and Pf-3 isolates respectively. These isolates were further confirmed by phenotypic characteristics. It is inferred from the present study, that taxonomical knowledge on bioagents is important for accurate identification and molecular characterization of potential biocontrol species. This is to undeniably avoid potential risk of introducing an unknown bacterial and fungal species into the rhizosphere of a given ecosystem. | Molecular characterization of potent biocontrol isolates of Trichoderma asperellum and Pseudomonas fluorescens from tomato rhizosphere

• Sinh học
• ITS region
• Phylogenetic analysis
• Tomato rhizosphere
• Pseudomonas fluorescens
• Trichoderma asperellum
• Molecular characterization
• Evaluate heavy metal pollution in the air obtained
• Moss technique in Hanoi and its surrounding region
• Barbula Indica moss
• Heavy metal elements
• Statistical analysis including principal component analysis
• Sclerotium rolfsii
• Ribosomal DNA Internal Transcribed Spacer (ITS) region
• Polymerase Chain Reaction
• Ribosomal DNA
• Mean score
• Farmers practicing organic farming
• Hill region
• Constraints faced
• Organic farming and its advantages
• Trichoderma isolates
• Tobacco growing regions
• Godavari district
• Based on sequence analysis
• Region of RDNA and morphological variations
• BMC Cancer
• Interval cancer rate
• Propotional incidence
• Girona Health Region
• Clinicopathological characteristics
• Climate change
• Adaptive capacity
• Vulnerability and Kosi region
• Comparative analysis of vulnerability indices
• Climatic vulnerability
• Decision making behaviour
• Social performance
• Economic performance
• Institutional participation
• Farm households in H K Region
• BMC Veterinary Research
• Multi locus sequence typing
• Mycoplasma bovis
• Genetic diversity
• Ningxia Hui Autonomous Region
• BMC Genetics
• Internal transcribed spacer
• Wedge clams
• Non transcribed spacer region
• Rhizoctonia solani
• Beans Phaseolus vulgaris L
• Genomic DNA
• DNA homology
• rDNA ITS
• Scientific reports
• scientific research
• biochemistry
• Structural features
• analysis of cytoplasmic
• hemoglobin
• trình bày báo cáo
• tài liệu báo cáo khoa học
• nghiên cứu y học
• báo cáo y học
• kiến thức y học
• Report medicine
• traditional medicine
• genetics
• Structural biochemistry
• Structural Biology
• molecular Biology
• tumor cells
• Antimicrobial
• cell structure
• DNA ITS phylogeny
• Phaseolus vulgaris
• Common bean
• Cymbocarpum alinihatii
• Phylogenetic position within Tordylieae
• Cymbocarpum species
• Allied species C
• anethoides
• C
• wiedemannii
• Socio economic impact
• MGNREGA beneficiaries
• Mahatma gandhi national rural
• Employment guarantee
• Selected villages
• Vanilla planifolia
• Fusarium oxysporum
• Root and stem rot
• Maximum likelihood
• Oxalic acid
• Volatile metabolites
• Trichoderma harzianum
• Camelina sativa
• Nitrogen dose
• Nitrogen scheduling
• Newly introduced biofuel crop
• Camelina sativa L
• Solanum lycopersicum L
• Yield and yield components
• Heterosis studies for yield
• Components in tomato
• Tomato (Solanum lycopersicum L )
• Zn fraction
• Rice wheat
• Rice wheat growing soils
• Distribution of zinc fractions
• Association with soil properties
• Machine transplanting
• Labour saving
• Comparative economics
• Cost of production
• Conventional method