TAILIEUCHUNG - Identification of Trichoderma isolates from tobacco growing regions of west Godavari district, based on sequence analysis of its region of RDNA and morphological variations in the strains

The aim of this study was to determine the phylogenetic relationships of Trichoderma isolates obtained from NLS Tobacco region. Utilizing the sequence analysis of internal transcribed spacer-1 (ITS-1) region of the ribosomal DNA the rDNA of Five Trichoderma isolates was amplified by polymerase chain reaction (PCR) using universal primers (ITS-1 and ITS-4). PCR products were purified and these purified products were used to amplify the ITS-4 region of the five Trichoderma isolates. The amplified DNA was sequenced and aligned against ex-type strain sequences from TrichoBLAST and established Trichoderma taxonomy. Two isolates were positively identified as Trichoderma harzianum and three isolates were identified as Trichoderma aesperulum which were used as an outgroup in these analyses. The ITS-1 region sequences have been used as the reference’s sequence and this could be further used for future study involving the identification and taxonomy of Trichoderma Amplification of ITS-1 region of the rDNA. This method proved as a rapid and reliable technique for identifying Trichoderma strains successfully. | Identification of Trichoderma isolates from tobacco growing regions of west Godavari district, based on sequence analysis of its region of RDNA and morphological variations in the strains

• Sinh học
• Trichoderma isolates
• Tobacco growing regions
• Godavari district
• Based on sequence analysis
• Region of RDNA and morphological variations
• Trichoderma viride
• Trichoderma viride isolates
• Different temperature
• Culture media
• Mycelia growth of Trichoderma viride isolates
• Biological control
• Pythium sp
• Different isolates of Trichoderma spp
• Management of damping
• Hydrolytic enzymes
• Trichoderma harzianum
• Trichoderma virens
• Soil borne pathogens
• Potential isolates of trichoderma species
• Seedling vigour
• In vitro compatibility
• Rhizobium and native trichoderma isolates
• Lentil rhizospheric soil
• Native trichoderma isolates
• Computational studies of Trichoderma
• Ribosomal RNA gene
• Commercial exploitation
• Economically important aerial
• Native trichoderma
• Rhizoctonia solani and antagonism
• Invitro evaluation
• Native rice specific isolates
• Trichoderma against rice
• Cultural and morphological characterization
• Morphological characterization
• Antagonistic Trichoderma Isolates
• Rhizospheric soil samples
• Cultural and morphological characteristics
• Colletotrichum graminicola
• Anthracnose of sorghum
• Culture filtrate
• Trichoderma isolates against
• Inhibition radial growth respectively
• Antagonistic capability
• Biopriming in vivo efficacy
• Trichoderrma etc
• Citrus fruit by local Trichoderma isolates
• Penicillium digitatum causing green rot
• Fusarium oxysporum
• Crown rot
• Aspergillus flavus
• Potential soil borne pathogens
• Local trichoderma isolates against
• Antagonistic action
• Fusarium oxysporum f sp lycopersci
• Studied microscopically
• Fusarium solaniisolates
• Trichoderma spp
• Root rot
• Papaya (Carica papaya L
• Commercial Trichoderma spp
• Collar rot
• Sclerotium rolfsii
• Trichoderma harzianum and vermicompost
• Collar rot of chickpea
• Sclerotium rolfsii Sacc
• Biocontrol potential of local isolates
• Native Trichoderma spp for the management
• Stem rot of groundnut caused
• In vitro antagonistic potential
• Pythium spp
• Rhizoctonia solani
• Per cent inhibition
• PGPR and biocontrol potential
• Solanum lycopersicum Miller
• ITS region
• Phylogenetic analysis
• Tomato rhizosphere
• Pseudomonas fluorescens
• Trichoderma asperellum
• Molecular characterization
• Pseudomonas sp
• Trichoderma sp
• Alternaria porri
• In vitro
• Pseudomonas fluorescens against Alternaria porri
• Abiotic stress
• Plant growth
• Rainfed condition in Bihar
• Stress tolerant rice varieties treated
• Secondary metabolites
• Antifungal compounds
• GC MS
• Retention time
• Pythium debaryanum
• Pseudomonas fluorescence
• Fungal isolates
• Charcoal rot
• Physiological parameters
• Disease incidence
• Physiological parameters of crop
• Cryphonectria parasitica
• Chestnut blight
• 5 Trichoderma sp
• 4 Penicillium sp
• Antagonistic organisms
• Fusarium oxysporum f sp
• Chickpea wilt
• Antagonistic variability
• In vitro condition
• Antagonistic ability
• Root knot nematodes
• Meloidogyne incognita
• Trichode rma isolates
• Tomato (Lycopersicon esculentum L Mill)
• Root knot nematode (Meloidogyne incognita)