TAILIEUCHUNG - Expression profiling of heat shock protein genes in two contrasting maize inbred lines

High temperature stress is one of the most detrimental abiotic stresses which adversely affect productivity of maize (Zea mays L.) in tropics and subtropics. Plants respond to high temperature stress by regulating expression of an array of genes, heat shock proteins (HSPs) being one of them. Owing to highly differential expression of HSPs in various crop species under high temperature stress, these could be considered as key stress responsive genes. Since HSPs gene family contain various members, identification of specific gene(s) playing crucial role in heat stress tolerance could be beneficial for developing stress resilient genotypes. Here we report in-silico characterization of five HSP genes and their expression analysis in two contrasting maize inbred lines . LM17 (heat tolerant) and HKI1015WG8 (heat susceptible) subjected to high temperature stress at seedling stage. The five maize specific HSP genes, viz., ZmHsp26, ZmHsp60, ZmHsp70, ZmHsp82 and ZmHsp101 exhibited distinctive expression pattern in response to heat stress. Higher upregulation of ZmHsp70 was found throughout the stress exposure in the heat tolerant line as compared to the susceptible line. Sharp up-regulation and rapid decline in expression of ZmHsp82 in LM17 than HKI1015WG8 after 12 hours heat stress exposure suggested its possible role in plant acclimatization to heat-stress conditions. Further, higher upregulation of ZmHsp101 even after removal of stress (recovery for 24 hrs) indicated its possible role in recovering plant from adverse effects of heat stress. The study opens up scope for investigation through transgenic (RNAi and/or over-expression) approach to further characterize and elucidate precise role of ZmHsp101, ZmHsp82 and ZmHsp70 in heat stress tolerance in maize. | Expression profiling of heat shock protein genes in two contrasting maize inbred lines

• Sinh học
• Heat shock proteins
• In silico analysis
• Real time PCR
• Heat tolerance
• Contrasting maize inbred lines
• BMC Pharmacology and Toxicology
• Caenorhabitis elegans
• Dopaminergic neurons
• BMC Molecular and Cell Biology
• Haliotis diversicolor
• Heat shock transcription factor 1
• Transcriptional regulation
• Irreversibly damaged proteins
• BMC Cancer
• Drug resistance
• Chemotherapeutic cytotoxic agents
• BMC Genomics
• Heat shock protein
• Regulatory motif
• Small heat shock proteins
• Ripening process
• Build multiple sequence motif
• Thermal stress
• Tasar silkworm
• Heat stress
• Tropical tasar silkworm
• BCLC stage
• Human hepatocellular carcinoma
• Hepatitis B virus
• Glucose regulated protein
• Breast cancer
• Differential gene expression
• Molecular subtypes
• HSP Clusts
• Rhizobia identification
• Molecular level stress response
• Oxidative stress response
• Plant Biology
• Ascorbic acid
• Hydrogen sulfide
• Nitrosative stress
• Oxidative stress
• Fragaria x ananassa
• BMC Veterinary Research
• Pathological injury
• Transport stress
• Ganxi goats
• Solanum lycopersicum L
• Abiotic stresses
• CD4 T cells
• CD8 T cells
• Bacterial heat shock proteins
• Aggregatibacter actinomycetemcomitans
• Chronic myeloid leukemia
• Client proteins
• Heat shock protein 90
• Heat shock protein 70
• Hybrid peptide
• Molecular chaperone
• BMC Pulmonary Medicine
• Idiopathic pulmonary fibrosis
• Single nucleotide polymorphisms
• Heat shock proteins of 70 kDa
• MHC polymorphisms
• Transgenic tobacco
• Electrolyte leakage
• DnaJ like protein
• Glucocorticoid receptor α
• Chaperoneheat shock proteins Hsp90
• MM cell
• Genome wide
• Hsp20 gene family
• Report medicine
• traditional medicine
• scientific reports
• medical research
• hemoglobin
• medical knowledge
• Crystal structure
• medical analysis
• biochemical studies
• BMC Plant Biology
• U box
• Protein transparent testa 12
• Plant photosynthetic apparatus
• UDP glycosyltransferase
• Structural biochemistry
• oxidation
• bacteria
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• environmental medicine
• cytoplasm
• tumor cells
• chemical reaction