TAILIEUCHUNG - Allele specific PCR based genotyping reveals lack of bovine leukocyte adhesion deficiency, bovine citrullinaemia and complex vertebral malformation carriers in Indian cattle population

Bovine Leukocyte Adhesion Deficiency (BLAD), Bovine Citrullinaemia (BC), and Complex Vertebral Malformation (CVM) are the common autosomal recessive disorders in cattle affecting dairy industry. The present study was designed to standardize allelespecific PCR based genotyping method for examining the occurrence of these disorders in Indian cattle population. A total of 327 indigenous (Bos indicus), exotic (HF) cattle breeds and HF crossbreds (Bos taurus × Bos indicus) from various parts of India were screened. An allele specific PCR technique was applied to detect mutation in specific DNA target sequences. The amplified products were purified and sequenced to confirm the change in nucleotide in the selected regions. Allele-specific PCR-based screening methodology was optimized for genotyping. No animals were found as carrier of these genetic abnormalities in the present study. Allele-specific PCR-based screening methodology may be efficiently employed to screen the animal population for these genetic defects. All tested animals were free from the aforementioned genetic disorders. But this may not provide us conclusively cattle herds maintained at the study area are free from these genetic disorders. Therefore, it is highly recommended to continue screening procedure to check undesirable transmission mutated version of alleles in Indian cattle population. | Allele specific PCR based genotyping reveals lack of bovine leukocyte adhesion deficiency, bovine citrullinaemia and complex vertebral malformation carriers in Indian cattle population

• Sinh học
• Genetic defects
• Genetic disorder
• Bovine citrullinaemia
• Allele specific PCR
• PCR based genotyping reveals lack
• Bovine leukocyte adhesion deficiency
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học
• Report medicine
• traditional medicine
• scientific reports
• biochemical studies
• environmental medicine
• Crystal structure
• Genetics in medicine
• Developmental genetics
• Birth defects
• Cancer genetics and genomics
• Risk assessment
• Genetic counseling
• Prenatal diagnosis and screening
• Personalized health care
• BMC Veterinary Research
• Corneal ulcers
• Precision medicine
• Ichthyoses encompass
• Severe hyperkeratosis
• BMC Pediatrics
• Conotruncal heart defect
• Single nucleotide polymorphism
• Methionine synthase
• Methylenetetrahydrofolate reductase
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo bệnh thú y
• kiến thức bênh thú y
• nghiên cứu bệnh thú y