TAILIEUCHUNG - Detection methods for seed borne pathogens

Seed is the custodian of the genetic, potential of any cultivar and determines the limits of productivity in any cropping system. So awareness for seed health has increased among the growers, traders and consumers in recent years. A seed-borne inoculum not only secures the presence of a virulent strain of the pathogen along the seed but also favors the earliest possible establishment of the infection in seedlings along with possible threat of introduction of new physiological races/stains with seed. With the guidelines of ISTA a number of detection methods starting from visual observation to PCR based molecular methods are being used for the detection of fungi, bacteria, virus and other seed borne pathogen. As detection of seed borne pathogens is the first line approach in managing seed borne diseases of plants so new methods of detection along with seed health testing approach are necessary to avoid the constraints in seed production and to reduce the cost for disease management in agriculture. | 2019 8 3 318-323 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 03 2019 Journal homepage http Review Article https Detection Methods for Seed Borne Pathogens Pooja Upadhyay1 and Samar Pal Singh2 . Pant Universty of Agriculture and Technology Pantnagar -263 145 India 2jc 7 Tf 1 AT 7 x 77 T 7- Krishi Vigyan Kendra New Delhi-73 India Corresponding author ABSTRACT Keywords Seed Borne Pathogens PCR based molecular method Article Info Accepted 04 February 2019 Available Online 10 March 2019 Seed is the custodian of the genetic potential of any cultivar and determines the limits of productivity in any cropping system. So awareness for seed health has increased among the growers traders and consumers in recent years. A seed-borne inoculum not only secures the presence of a virulent strain of the pathogen along the seed but also favors the earliest possible establishment of the infection in seedlings along with possible threat of introduction of new physiological races stains with seed. With the guidelines of ISTA a number of detection methods starting from visual observation to PCR based molecular methods are being used for the detection of fungi bacteria virus and other seed borne pathogen. As detection of seed borne pathogens is the first line approach in managing seed borne diseases of plants so new methods of detection along with seed health testing approach are necessary to avoid the constraints in seed production and to reduce the cost for disease management in agriculture. Introduction Seed is the custodian of the genetic potential of the cultivar thus the quality of the seed determines the limits of productivity to be realized in any given cropping system. In recent years the awareness for seed health has increased among the growers traders and consumers especially post GATT era and with the emergence of WTO .

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