TAILIEUCHUNG - Molecular detection and characterization of Niger [Guizotia abyssinica (L.f.) Cass] phyllody phytoplasma

Niger [Guizotia abyssinica (.) Cass.] is one of the important minor oilseed crops in India. Phyllody disease on niger noticed for the first time at Main Research Station, Hebbal, Bengaluru, Karnataka. The molecular detection and characterization of the phytoplasma causing niger phyllody was investigated during Kharif 2016. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by polymerase chain reaction (PCR) from infected samples, as well as by sequencing and phylogenetic analysis. The molecular detection by using nested PCR phytoplasma specific universal primers R16F2n/R16FR2 revealed the amplification of phytoplasmal specific PCR product of kb fragment corresponding to the 16S rDNA. The16S rDNA sequence of niger phyllody phytoplasma had maximum nucleotide identity of 90 per cent with the 16S rDNA sequence of Cymbopogon citratus white leaf phytoplasma (), Alfalfa witches'-broom Phytoplasma Mes 38(), Sesamum indicum phyllody Phytoplasma () and Vigna radiata phyllody NDL (). | Molecular detection and characterization of Niger [Guizotia abyssinica (.) Cass] phyllody phytoplasma

• Sinh học
• Molecular detection
• Phyllody phytoplasma
• Characterization of Niger
• Cymbopogon citratus
• Leaf phytoplasma
• Prevalence and molecular detection
• Blood protozoa
• Domestic pigeon
• Haemoprotozoan infection
• Brucella spp
• Molecular detection of Brucella spp
• Reproductive disorders
• Vaginal mucus
• Brucella abortus
• Real time PCR
• Molecular detection of Brucella abortus
• Buffaloes in Gujarat
• Japanese encephalitis
• Pigs and punjab
• Serological and molecular detection
• Molecular detection of japanese encephalitis
• Naturally infected pigs
• Molecular protocol
• Establishing a molecular protocol
• Detection of EGFR mutations
• Non small cell lung cancer
• The molecular protocol constructed
• Multidrug resistant
• Molecular detection of Rifampicin
• Molecular detection of Isoniazid
• Mycobacterium Tuberculosis complex
• Using line probe assay
• Molecular detection of brucella organism
• Brucella organism
• Brucella organism from milk
• Milk products
• Ehrlichia canis
• Nested PCR
• Canine monocytic ehrlichiosis
• Molecular detection of canine monocytic ehrlichiosis
• Pathomorphological and molecular detection
• Black spot
• Asperisporium caricae
• Incited byasperisporium caricae
• Detection and molecular characterization
• Virusfree garlic
• Bulb associated potyviruses in garlic
• Field response
• Molecular detection of bulb associated potyviruses
• Garlic through molecular diagnosis
• BMC Veterinary Research
• Pig brucellosis
• Molecular evidence
• Systematically quantify and identify
• Molecular techniques
• Listeria monocytogenes
• Antibiotic sensitivity
• Antibiotic sensitivity profile
• Cattle sources of aizawl
• Ziehl Neelsen stain
• Rapid molecular detection
• Mycobacterium tuberculosis
• Comparison to other conventional methods
• Conventional methods
• Polymerase chain reaction assays
• Brucella abortus from cattle
• Brucella abortus from buffalo
• DNA extraction
• Major streptococcus spp
• Antimicrobial resistance
• Bovine mastitis
• Antimicrobial resistance pattern
• Vibrio alginolyticus
• Isothermal amplification
• Recombinase polymerase amplification
• Lateral flow dipstick
• Clinico seroepidemiology
• Molecular characterization of brucellosis
• Brucellosis in animals
• Brucella antibodies by RBPT
• Chili pepper
• Molecular marker
• Resistant chili pepper cultivars
• Anthracnose resistance associated molecular markers
• Resistant chili pepper cultivars in Vietnam
• Cinnamon witches’ broom
• Identification of a phytoplasma associated
• Cinnamomum cassia B
• Khasi mandarin
• Candidatus liberibacter asiaticus
• Khasi mandarin orchards
• Total DNA was extracted
• Papaya ring spot virus
• Papaya ring spot virus disease
• NCBI Genebank
• PRSV in the plants
• Virulence associated genes
• Salmonella Serovars isolated
• Raw pork of Aizawl and Imphal
• Worry from hygienic point
• China aster
• Molecular and detection
• Characterization of china aster
• 16S rII phytoplasmal group
• Latex agglutination test
• Group a rotaviruses
• Bhandara and Chandrapur district
• Circulating rotaviruses