TAILIEUCHUNG - Establishing a molecular protocol for detection of EGFR mutations in patients with non-small cell lung cancer

Lung cancer is one of the quickest and most fatal diseases and is divided into two types: small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). Approximately 50% of NSCLC patients harbour mutations in the epidermal growth factor receptor (EGFR) gene, spanning from exon 18 to exon 21, which are responsible for the tyrosine kinase domain of the EGFR protein. Currently, gefitinib and erlotinib are two drugs inhibiting EGFR which are used in the treatment of lung cancer. The indication for use of these drugs depends on the mutation status of EGFR. Resistance to treatment with these two drugs has been found with mutations in exon 20; however, mutations in exon 18, 19, 21 benefited from the same treatment. Therefore, we built a molecular protocol for detecting mutations in exon 21 of EGFR in NSCLC patients in Vietnam which, in this study, is based on the realtime polymerase chain reaction high resolution melting (PCR HRM) technique. We successfully designed pairs of primers for the real-time PCR HRM technique to detect mutations in exon 21 and for the Sanger sequencing method to confirm the real-time PCR HRM results. We also investigated the optimal concentration of MgCl2 for the real-time PCR HRM protocol. Performance characteristics of the real-time PCR HRM protocol were evaluated in terms of specificity, limit of detection, repeatability, and it showed good results. Finally, we applied the molecular protocol to 172 clinical samples and compared these with the Sanger nucleotide sequencing method and the peptide nucleic acid (PNA) clamping method. The results of the comparison demonstrate that the molecular protocol constructed for detection of exon 21 mutations can be used in the treatment of NSCLC patients. | Establishing a molecular protocol for detection of EGFR mutations in patients with non-small cell lung cancer

• Y khoa - Dược
• Molecular protocol
• Establishing a molecular protocol
• Detection of EGFR mutations
• Non small cell lung cancer
• The molecular protocol constructed
• Heavy Metal Toxicity
• Genetic and Molecular
• Analysis of Differential
• Genes of Uyghur
• Women with Endometriosis
• Xinjiang
• BMC Molecular and Cell Biology
• Molecular docking
• Protein ligand docking
• Protein peptide docking
• Conformational sampling
• Incremental protocol
• Commiphora wightii
• Molecular markers
• PCR amplification
• Polyvinyl pyrrolidone
• DNA extraction
• BMC Cancer
• Psychological resilience
• Body and mind
• Biomolecular parameters
• Breast cancer
• Quality of life
• Lymphatic system
• Manual lymphatic drainage
• Molecular imaging
• Tumour genomic profiling
• Genome sequencing
• Germline sequencing
• Molecular profiling
• Psychosocial factors
• Health behaviors
• Precision medicine
• Cervical cancer
• Reverse phase protein array
• Tumor micro environment
• Targeted therapy
• Patient stratification
• BMC Pulmonary Medicine
• Ventilator associated pneumonia
• Breath analysis
• Volatile organic compounds
• Empiric antibiotic treatment
• Porcine heart
• Endothelial cell
• Fresh tissue
• Cardiovascular studies
• Anaplastic lymphoma kinase
• Tyrosine kinase inhibitors
• Resistance mutations
• Molecular risk
• Virtual molecular tumor board
• Next generation sequencing
• Mass spectrometry
• Journal of Botany
• Termitomyces sheikhupurensis sp nov
• DNA sequences data
• New symbiotic species
• Morphological protocols