TAILIEUCHUNG - A simple and efficient genomic DNA extraction protocol for dried leaf of threatened species Commiphora Wightii (Arnott) Bhandari for genetic analysis of plant biological system

Commiphora wightii (Arnott) Bhandari contains secondary metabolites, polysaccharides and phenolic compounds. The presence of secondary metabolites, reduce the yield and quality of the DNA. In the present study an alternative protocol for genomic DNA extraction from dry plant leaves was developed that is acquiescent to PCR-based genomic studies. Existing protocols were lengthy, costly or not appropriate for genomic DNA extraction from dry leaves. This modified CTAB (3%) and PVP (Polyvinylpyrrolidone) protocol include the use of NaCl, ß-mercaptoethanol in the extraction as well as application of autoclaved sand for proper grinding of dried leaves and inclusion of RNase A treatment in the protocol to fasten the process. The extracted DNA using present optimized protocol was super in quality and quantity. It was also suitable for polymerase chain reaction with random decamer, inter simple sequence repeat and barcode primers. The developed protocol is rapid and cost efficient with high quality and sufficient quantity of DNA for downstream PCR-based genetic analysis. | A simple and efficient genomic DNA extraction protocol for dried leaf of threatened species Commiphora Wightii (Arnott) Bhandari for genetic analysis of plant biological system

• Sinh học
• Commiphora wightii
• Molecular markers
• PCR amplification
• Polyvinyl pyrrolidone
• DNA extraction
• Asexual morphological differences in male
• Asexual morphological differences in female plants
• Commiphora wightii Bhandari
• An endangered medicinal plant
• Leaf characters viz
• Nodal segment
• Shoot apex
• In vitro
• In vitro culture of Guggul
• Commiphora wightii Arnott
• Callus induction
• Tissue culture
• Invitro conditions
• Explants of Guggul wightii
• Hibiscus cannabinus
• Anethum gravelons
• Emblica officinalis
• Ficus spp
• Mentha arvensis
• Mimusops elengi
• Antibacterial activity
• Simple genomic DNA extraction
• Efficient and universal method
• The extraction of genomic DNA
• Genomic DNA from bacteria
• Microalgae suitable
• PCR based applications
• Pigeon pea
• Genomic DNA extraction
• Genomic DNA extraction from pigeon pea
• Rapid procedure
• Showed highly pure DNA
• Vietnam Journal of Science and Technology
• Optimizing dna extraction condition
• Optimizing dna extraction condition from wood
• Response surface methods
• Response Surface Methodology
• Oryza sativa
• Solanum lycopersicum
• Genomic DNA
• DNA purity
• Green gram
• Liquid nitrogen
• Mungbean without liquid nitrogen
• High quality DNA extraction
• Ancient DNA
• Mainland serow
• Sequencing method
• Modern human DNA
• Rapid and efficient procedure
• Trichoderma spp
• DNA with high yield
• PCR purposes
• DNA extraction method
• Dioscorea esculenta
• Quality of DNA
• commercial kit
• Lesser yam (Dioscorea esculenta)
• Clinical Specimens
• Polymerase chain reaction
• Nucleic acid extraction
• Anti microbial resistance
• Deoxyribonucleic acid
• Cetyl trimethyl ammonium bromide
• Comparison of phenol chloroform
• Carbohydrate polymers
• Magnetic hybrid nanocomposite
• Human blood
• UV–vis absorption
• DNA BIOLOGY
• COMMERCIAL KITS
• STUTTER PRODUCTS
• DNA ANALYSIS
• MICROBIAL FORENSICS
• Cyprinid fishes
• Formalin preserved tissue
• Mitochondrial DNA
• Quality analysis
• Distribution and phylogenetic
• Relationships of snappers
• Phylogenetic relationships of snappers
• DNA sequence variation of the mitochondrial
• DNA isolation
• Phylogenetic analysis
• Molecular characterization of the pathogen
• Good quality fungal DNA directly
• Infected banana
• Rapid and efficient
• Extraction protocol
• Isolation of fungal DNA
• Fungal DNA
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• kiến thức y học
• nghiên cứu y học
• BMC Bioinformatics
• K mer
• Next generation sequencing
• De novo assembly
• Web server
• Redundancy control
• Quality control
• Protein coding DNA sequences
• Sequence databases increases
• BMC Genomics
• DNA sequencing
• Target sequencing panel
• Formalin fixed tissue
• Breast tissue
• Mutational signature
• Molecular barcode
• African swine fever virus
• Direct PCR
• Mutant DNA polymerase
• PCR inhibitors whole blood
• Epiretinal Prostheses
• Cell Lysis
• Measuring Methods
• MEMS Devices
• Coplanar Waveguide
• Sex determination
• Bronze Age
• Oylum Höyük
• Genetic methods
• Penaeid shrimp
• Early mortality syndrome
• Acute hepatopancreatic necrosis disease
• Bacterial diversity
• Bacterial DNA extraction
• Bacteria specific library construction