TAILIEUCHUNG - Identification of candidate gene based markers (SNPs) in the ZIP family transporter sequences of rice (Oryza sativa L.)

Iron and Zinc are the key ingredient in human nutrition, soil and crop management. Rice is the major dietary source for the population in India but it is deficient in iron and zinc. An effort was made to quantify the iron and zinc content in brown, white and cooked white rice and to study the effect of nucleotide variations in genes associated with zinc. 14 genotypes were selected and grown in field at UAS, GKVK in RCBD, during Kharif-2014. Quality parameter zinc in brown rice showed negative effect on yield. Highly positively significant correlation was observed for grain yield with number of productive tillers. Literature survey revealed genes belonging to transporter family OsZIP are involved in transporting zinc. Primers were designed specific to these genes. The primer profile at gel level was monomorphic for all the primers. Sequencing the amplicons of primersOsZIP2-1 and OsZIP7-2 revealed that variations were present in all these amplicons but nucleotide changes at ninth position of OsZIP2-1 were significantly associated with brown rice zinc content and nucleotide changes at 25, 40 and 551 positions of OsZIP7-2 were significantly associated with grain iron content. Although OsZIP2-1 and OsZIP7-2 were monomorphic at gel level nucleotide variations were significantly associated with grain iron and zinc content. The markers identified in this study can be used for improving the grain iron and zinc in rice. | Identification of candidate gene based markers (SNPs) in the ZIP family transporter sequences of rice (Oryza sativa L.)

• Sinh học
• Candidate gene
• ZIP Family
• Oryza sativa
• Candidate gene based markers
• Family transporter sequences of rice
• Oryza sativa L
• Candidate disease gene prediction
• Candidate disease genes
• Mendelian diseases
• Complex diseases
• Genome wide association studies
• Candidate gene identification
• BMC Plant Biology
• Salt tolerance
• Salt responsive gene
• Molecular diversity
• Rice genotype
• BMC Pulmonary Medicine
• Neuropeptide S receptor 1
• Asthma candidate gene
• Allergy susceptibility gene
• Pathogenetic processes
• Designing functional markers
• The candidate gene SRWD2 involved
• Salt tolerances
• Vietnamese rice landraces
• Develop salt tolerance in rice varieties
• Rice aluminum tolerance
• Genome wide association study
• Transcriptomic analysis
• Quantitative trait locus
• Candidate gene prioritization
• Gene function prediction
• Differential gene expression
• RNA Seq
• Real time PCR
• BMC Bioinformatics
• Anatomy ontology
• Data integration
• Candidate gene prediction
• Data quality
• Semantic similarity
• BMC Genomics
• Maintaining high biomass
• Glycine max L
• Soybean yield
• Gibberella ear rot
• Fusarium graminearum
• Gene expression
• Disease resistance
• Fungal pathogen
• Gene regulatory networks
• Bayesian network
• Candidate auto selection
• Breakpoint detection
• Search space reduction
• Dairy cattle
• Female fertility
• Gene base analysis
• Gene annotation
• Genetic marker
• Polymorphism vis a vis immune response
• Important infectious diseases
• Genetic gain is cumulative
• Chronic obstructive pulmonary disease
• Expression information
• Protein–protein interaction networks
• Re sequencing
• Candidate genes
• Physiological factors
• Adequately understood
• Ear rot
• Fusarium verticillioides
• Near isogenic lines
• Genome wide association analysis
• Hundred seed weight
• Single nucleotide polymorphism
• BMC Genetics
• Genetic effect
• Milk composition traits
• Differentially expressed
• Potential candidate genes
• Heterotypic cell cell adhesion
• RNA sequenced
• RNA sequencing
• Milk production traits
• Candidate gene approach
• Cytosine adenine microsatellite polymorphism
• IGF1 gene
• Age at natural menopause
• Age at menarche
• Smoking status
• Cf 19 gene
• CSolanum lycopersicum
• Cladosporium fulvum
• Tomato leaf mold
• SLAF seq
• BMC Cancer
• Head and neck squamous cell carcinoma
• Gene amplification
• Genomic aberrations
• TRPC6 gene
• Johne’s disease
• Interleukin 10 receptor alpha gene
• Gene knockout