TAILIEUCHUNG - Effect of YAP inhibition on human leukemia HL-60 cells

Yes-associated protein (YAP), the nuclear effector of the Hippo pathway, is a candidate oncoprotein and participates in the progression of various malignancies. However, few reports have examined the effect of YAP inhibition in human leukemia HL-60 cells. | Int. J. Med. Sci. 2017 Vol. 14 902 ivyspring international publisher International Journal of Medical Sciences 2017 14 9 902-910. doi Research Paper Effect of YAP Inhibition on Human Leukemia HL-60 Cells Min Chen1 2 Jian Wang2 Shi-Fei Yao1 2 Yi Zhao1 2 Lu Liu2 Lian-Wen Li1 2 Ting Xu1 2 Liu-Gen Gan1 2 Chun-Lan Xiao1 2 Zhi-Ling Shan2 Liang Zhong2E Bei-Zhong Liu1 - 1. Central Laboratory of Yong-chuan Hospital Chongqing Medical University Chongqing 402160 China 2. Key Laboratory of Laboratory Medical Diagnostics Ministry of Education Department of Laboratory Medicine Chongqing Medical University Chongqing 400016 China El Corresponding authors Liang Zhong Key Laboratory of Laboratory Medical Diagnostics Ministry of Education Department of Laboratory Medicine Chongqing Medical University 1 Yixueyuan Road Chongqing 400016 China. Tel 86 13637931208 E-mail cnmed1@ or Bei-Zhong Liu Department of Laboratory Medicine Chongqing Medical University 1 Yixueyuan Road Chongqing 400016 China. Tel 86 18716474304 Fax 86 023-68485006 E-mail liubeizhong@ Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution CC BY-NC license https licenses by-nc . See http terms for full terms and conditions. Received Accepted Published Abstract Background Yes-associated protein YAP the nuclear effector of the Hippo pathway is a candidate oncoprotein and participates in the progression of various malignancies. However few reports have examined the effect of YAP inhibition in human leukemia HL-60 cells. Methods We examined the effects of YAP knockdown or inhibition using short hairpin RNA shRNA or verteporfin VP respectively. Western blot assays were used to determine the expression levels of YAP Survivin cyclinD1 PARP Bcl-2 and Bax. Cell proliferation was assessed using the cell counting kit CCK-8 assay. Cell cycle .

• Y khoa - Dược
• Yes associated protein
• Human leukemia HL 60 cells
• YAP inhibition
• Hippo pathway
• Cell nuclear fragmentation