TAILIEUCHUNG - Developmental stages of ovule and megagametophyte in Chenopodium botrys L. (Chenopodiaceae)

The ovule ontogenesis and the megasporogenesis stages in Chenopodium botrys L. were studied with light microscopy. Flowers and young pods were removed from natural plants and fixed in FAA 70, stored in 70% ethanol, embedded in paraffin, and sectioned at 7 μm with a microtome. Staining was carried out with Hematoxylin and Eosin and developmental stages of ovule were studied. | Research Article Turk J Bot 33 (2009) 75-81 © TÜBİTAK doi: Developmental Stages of Ovule and Megagametophyte in Chenopodium botrys L. (Chenopodiaceae) Abdolkarim CHEHREGANI1,*, Behrouz MALAYERI1, Nafiseh YOUSEFI2 1 Department of Biology, Bu-Ali Sina University, Hamedan - IRAN 2 Colleges of Basic Science, Islamic Azad University, Hamedan - IRAN Received: Accepted: Abstract: The ovule ontogenesis and the megasporogenesis stages in Chenopodium botrys L. were studied with light microscopy. Flowers and young pods were removed from natural plants and fixed in FAA 70, stored in 70% ethanol, embedded in paraffin, and sectioned at 7 μm with a microtome. Staining was carried out with Hematoxylin and Eosin and developmental stages of ovule were studied. The results of this research showed that ovule development, including megasporogenesis and initial stages of megagametogenesis, occurred while flowers were still in bud. In C. botrys the female gametophyte has a monosporic origin and the developmental pattern exhibited by this species is referred to as the polygonum type. Development of ovule starts with the formation of a primordium. In this primordium, an archesporial cell produces a megaspore mother cell, which undergoes meiosis, forming a linear tetrad. The micropylar cell is a functional megaspore that survives and will function in megagametophyte development. The mature gametophyte is composed of 7 cells: 1 secondary nucleus, 2 synergids, 1 egg cell, and 3 antipodal cells. Key Words: Chenopodium botrys, embryo sac, megagametogenesis, megasporogenesis, ovule development, Chenopodiaceae Introduction Chenopodiaceae family is a member of Centrospermae, which covers about 100 genera and 1500 species (Datta, 2003). The members of this family are distributed chiefly in Australia, the Karro (South Africa), the Red Sea shores, the South-West Caspian coast, Central Asia, and the salt steppes of East Asia. Most of the Chenopodiaceae

• Nông nghiệp
• Chenopodium botrys
• Rmbryo sac
• Ovule development
• Light microscopy
• Antipodal cells
• Scilla autumnalis
• S
• autumnalis
• Cytochemical features
• Nucellar epidermal cells
• Chenopodium botrys L
• Megaspore mother cell
• Central cell
• Female gametophyte
• Polar nucleus
• 3 antipodal cells
• 2 synergids